New genotypes of Liao ning virus (LNV) in Australia exhibit an insect-specific phenotype

Prow, Natalie A., Mah, Marcus G., Deerain, Joshua M., Warrilow, David, Colmant, Agathe M. G., O'Brien, Caitlin A., Harrison, Jessica J., McLean, Breeanna J., Hewlett, Elise K., Piyasena, Thisun B. H., Hall-Mendelin, Sonja, van den Hurk, Andrew F., Watterson, Daniel, Huang, Bixing, Schulz, Benjamin L., Webb, Cameron E., Johansen, Cheryl A., Chow, Weng K., Hobson-Peters, Jody, Cazier, Chris, Coffey, Lark L., Faddy, Helen M., Suhrbier, Andreas, Bielefeldt-Ohmann, Helle and Hall, Roy A. (2018) New genotypes of Liao ning virus (LNV) in Australia exhibit an insect-specific phenotype. The Journal of General Virology, 99 4: 596-609. doi:10.1099/jgv.0.001038


Author Prow, Natalie A.
Mah, Marcus G.
Deerain, Joshua M.
Warrilow, David
Colmant, Agathe M. G.
O'Brien, Caitlin A.
Harrison, Jessica J.
McLean, Breeanna J.
Hewlett, Elise K.
Piyasena, Thisun B. H.
Hall-Mendelin, Sonja
van den Hurk, Andrew F.
Watterson, Daniel
Huang, Bixing
Schulz, Benjamin L.
Webb, Cameron E.
Johansen, Cheryl A.
Chow, Weng K.
Hobson-Peters, Jody
Cazier, Chris
Coffey, Lark L.
Faddy, Helen M.
Suhrbier, Andreas
Bielefeldt-Ohmann, Helle
Hall, Roy A.
Title New genotypes of Liao ning virus (LNV) in Australia exhibit an insect-specific phenotype
Journal name The Journal of General Virology   Check publisher's open access policy
ISSN 1465-2099
0022-1317
Publication date 2018-04-01
Year available 2018
Sub-type Article (original research)
DOI 10.1099/jgv.0.001038
Open Access Status Not yet assessed
Volume 99
Issue 4
Start page 596
End page 609
Total pages 14
Place of publication London, United Kingdom
Publisher The Microbiology Society
Language eng
Subject 2406 Virology
Abstract Liao ning virus (LNV) was first isolated in 1996 from mosquitoes in China, and has been shown to replicate in selected mammalian cell lines and to cause lethal haemorrhagic disease in experimentally infected mice. The first detection of LNV in Australia was by deep sequencing of mosquito homogenates. We subsequently isolated LNV from mosquitoes of four genera (Culex, Anopheles, Mansonia and Aedes) in New South Wales, Northern Territory, Queensland and Western Australia; the earliest of these Australian isolates were obtained from mosquitoes collected in 1988, predating the first Chinese isolates. Genetic analysis revealed that the Australian LNV isolates formed two new genotypes: one including isolates from eastern and northern Australia, and the second comprising isolates from the south-western corner of the continent. In contrast to findings reported for the Chinese LNV isolates, the Australian LNV isolates did not replicate in vertebrate cells in vitro or in vivo, or produce signs of disease in wild-type or immunodeficient mice. A panel of human and animal sera collected from regions where the virus was found in high prevalence also showed no evidence of LNV-specific antibodies. Furthermore, high rates of virus detection in progeny reared from infected adult female mosquitoes, coupled with visualization of the virus within the ovarian follicles by immunohistochemistry, suggest that LNV is transmitted transovarially. Thus, despite relatively minor genomic differences between Chinese and Australian LNV strains, the latter display a characteristic insect-specific phenotype.
Keyword Australia
Liao ning virus
Host restriction
Mosquito-borne
Seadornavirus
Q-Index Code C1
Q-Index Status Provisional Code
Grant ID ARC DP120103994
Institutional Status UQ

 
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Created: Wed, 21 Mar 2018, 10:07:48 EST