B cell chronic lymphocytic leukaemia cells have reduced capacity to upregulate expression of MHC class I in response to interferon-gamma

Juffs, Helen, Fowler, Nina, Saal, Russell, Grimmett, Karen, Beasley, Shannon, O'Sullivan, Brendan, Frazer, Ian H., Gill, Devinder and Thomas, Ranjeny (2004) B cell chronic lymphocytic leukaemia cells have reduced capacity to upregulate expression of MHC class I in response to interferon-gamma. Pathology, 36 1: 69-76. doi:10.1080/00313020310001644499


Author Juffs, Helen
Fowler, Nina
Saal, Russell
Grimmett, Karen
Beasley, Shannon
O'Sullivan, Brendan
Frazer, Ian H.
Gill, Devinder
Thomas, Ranjeny
Title B cell chronic lymphocytic leukaemia cells have reduced capacity to upregulate expression of MHC class I in response to interferon-gamma
Journal name Pathology   Check publisher's open access policy
ISSN 0031-3025
1465-3931
Publication date 2004-02-01
Year available 2004
Sub-type Article (original research)
DOI 10.1080/00313020310001644499
Open Access Status Not yet assessed
Volume 36
Issue 1
Start page 69
End page 76
Total pages 8
Editor C. S. Lee
Place of publication Basingstoke
Publisher Carfax/Taylor & Francis Ltd
Language eng
Subject C1
320206 Tumor Immunology
730103 Blood disorders
110322 Rheumatology and Arthritis
1107 Immunology
Abstract Aims: An important consideration in the design of a tumour vaccine is the ability of tumour-specific cytotoxic T lymphocytes (CTL) to recognise unmanipulated tumour cells in vivo. To determine whether B-CLL might use an escape strategy, the current studies compared B-CLL and normal B cell MHC class I expression. Methods: Flow cytometry, TAP allele PCR and MHC class I PCR were used. Results: While baseline expression of MHC class I did not differ, upregulation of MHC class I expression by B-CLL cells in response to IFN-gamma was reduced. No deletions or mutations of TAP 1 or 2 genes were detected. B-CLL cells upregulated TAP protein expression in response to IFN-gamma. Responsiveness of B-CLL MHC class I mRNA to IFN-gamma was not impaired. Conclusions: The data suggest that MHC class I molecules might be less stable at the cell surface in B-CLL than normal B cells, as a result of the described release of beta(2)m and beta(2)m-free class I heavy chains from the membrane. This relative MHC class I expression defect of B-CLL cells may reduce their susceptibility to CTL lysis in response to immunotherapeutic approaches.
Keyword Human
B-cll
Antigen Presentation
Mhc Class I
Tap
Antigen-processing Machinery
Cervical Carcinomas
Ifn-gamma
Transporter Protein
Immune Recognition
Tap-1 Expression
Down-regulation
Hla Expression
Gene-transfer
Lung-cancer
Pathology
Q-Index Code C1
Institutional Status UQ

 
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Created: Wed, 15 Aug 2007, 14:11:12 EST