Isolation and characterization of integron-containing bacteria without antibiotic selection

Barlow, Robert S., Pemberton, John M., Desmarchelier, Patricia M. and Gobius, Kari S. (2004) Isolation and characterization of integron-containing bacteria without antibiotic selection. Antimicrobial Agents And Chemotherapy, 48 3: 838-842. doi:10.1128/AAC.48.3.838-842.2004

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Author Barlow, Robert S.
Pemberton, John M.
Desmarchelier, Patricia M.
Gobius, Kari S.
Title Isolation and characterization of integron-containing bacteria without antibiotic selection
Journal name Antimicrobial Agents And Chemotherapy   Check publisher's open access policy
ISSN 0066-4804
Publication date 2004-03-01
Sub-type Article (original research)
DOI 10.1128/AAC.48.3.838-842.2004
Open Access Status File (Publisher version)
Volume 48
Issue 3
Start page 838
End page 842
Total pages 5
Editor G. Eliopoulos
Place of publication Washington, DC, United States
Publisher American Society for Microbiology
Language eng
Subject C1
320401 Medical Bacteriology
730216 Food safety
270201 Gene Expression
780105 Biological sciences
Abstract The emergence of antibiotic resistance among pathogenic and commensal bacteria has become a serious problem worldwide. The use and overuse of antibiotics in a number of settings are contributing to the development of antibiotic-resistant microorganisms. The class 1 and 2 integrase genes (intI1 and intI2, respectively) were identified in mixed bacterial cultures enriched from bovine feces by growth in buffered peptone water (BPW) followed by integrase-specific PCR. Integrase-positive bacterial colonies from the enrichment cultures were then isolated by using hydrophobic grid membrane filters and integrase-specific gene probes. Bacterial clones isolated by this technique were then confirmed to carry integrons by further testing by PCR and DNA sequencing. Integron-associated antibiotic resistance genes were detected in bacteria such as Escherichia coli, Aeromonas spp., Proteus spp., Morganella morganii, Shewanella spp., and urea-positive Providencia stuartii isolates from bovine fecal samples without the use of selective enrichment media containing antibiotics. Streptomycin and trimethoprim resistance were commonly associated with integrons. The advantages conferred by this methodology are that a wide variety of integron-containing bacteria may be simultaneously cultured in BPW enrichments and culture biases due to antibiotic selection can be avoided. Rapid and efficient identification, isolation, and characterization of antibiotic resistance-associated integrons are possible by this protocol. These methods will facilitate greater understanding of the factors that contribute to the presence and transfer of integron-associated antibiotic resistance genes in bacterial isolates from red meat production animals.
Keyword Microbiology
Pharmacology & Pharmacy
Class-i Integrons
Gene Cassettes
Q-Index Code C1

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Created: Wed, 15 Aug 2007, 14:10:29 EST