The use of SWATH to analyse the dynamic changes of bacterial proteome of carbapanemase-producing Escherichia coli under antibiotic pressure

Sidjabat, Hanna E., Gien, Jolene, Kvaskoff, David, Ashman, Keith, Vaswani, Kanchan, Reed, Sarah, McGeary, Ross P., Paterson, David L., Bordin, Amanda and Schenk, Gerhard (2018) The use of SWATH to analyse the dynamic changes of bacterial proteome of carbapanemase-producing Escherichia coli under antibiotic pressure. Scientific Reports, 8 1: 3871. doi:10.1038/s41598-018-21984-9


Author Sidjabat, Hanna E.
Gien, Jolene
Kvaskoff, David
Ashman, Keith
Vaswani, Kanchan
Reed, Sarah
McGeary, Ross P.
Paterson, David L.
Bordin, Amanda
Schenk, Gerhard
Title The use of SWATH to analyse the dynamic changes of bacterial proteome of carbapanemase-producing Escherichia coli under antibiotic pressure
Journal name Scientific Reports   Check publisher's open access policy
ISSN 2045-2322
Publication date 2018-03-01
Year available 2018
Sub-type Article (original research)
DOI 10.1038/s41598-018-21984-9
Open Access Status DOI
Volume 8
Issue 1
Start page 3871
Total pages 11
Place of publication London, United Kingdom
Publisher Nature Publishing Group
Language eng
Subject 1000 General
Abstract Antibiotic resistance associated with the clinically significant carbapenemases KPC, NDM and OXA-48 in Enterobacteriaceae is emerging as worldwide. In Australia, IMP-producing Enterobacteriaceae are the most prevalent carbapenemase-producing Enterobacteriaceae (CPE). Genomic characteristics of such CPE are well described, but the corresponding proteome is poorly characterised. We have thus developed a method to analyse dynamic changes in the proteome of CPE under antibiotic pressure. Specifically, we have investigated the effect of meropenem at sub-lethal concentrations to develop a better understanding of how antibiotic pressure leads to resistance. Escherichia coli strains producing either NDM-, IMP- or KPC-type carbapenemases were included in this study, and their proteomes were analysed in growth conditions with or without meropenem. The most significant difference in the bacterial proteomes upon the addition of meropenem was triggered amongst NDM-producers and to a lower extent amongst KPC-producers. In particular, HU DNA-binding proteins, the GroEL/GroES chaperonin complex and GrpE proteins were overexpressed. These proteins may thus contribute to the better adaptability of NDM- and KPC-producers to meropenem. A significant meropenem-induced increase in the expression of the outer membrane protein A was only observed in IMP-producers, thus demonstrating that carbapenemase-mediated resistance relies on far more complex mechanisms than simple inactivation of the antibiotic.
Keyword Metallo-Beta-Lactamase
Klebsiella-Pneumoniae
Pseudomonas-Aeruginosa
Carbapenem Resistance
Enterobacter-Cloacae
Genetic Contexts
In-Vitro
Identification
Inhibitors
Dna
Q-Index Code C1
Q-Index Status Provisional Code
Grant ID FT120100694
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: UQ Centre for Clinical Research Publications
HERDC Pre-Audit
Faculty of Medicine
School of Chemistry and Molecular Biosciences
 
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Created: Wed, 07 Mar 2018, 11:04:02 EST