Effect after introducing Bacillus thuringiensis gene on nitrogen metabolism in cotton

Chen, D. H., Ye, G. Y., Yang, C. Q., Chen, Y. and Wu, Y. K. (2004) Effect after introducing Bacillus thuringiensis gene on nitrogen metabolism in cotton. Field Crops Research, 87 2-3: 235-244. doi:10.1016/j.fcr.2003.11.001


Author Chen, D. H.
Ye, G. Y.
Yang, C. Q.
Chen, Y.
Wu, Y. K.
Title Effect after introducing Bacillus thuringiensis gene on nitrogen metabolism in cotton
Journal name Field Crops Research   Check publisher's open access policy
ISSN 0378-4290
Publication date 2004-01-01
Sub-type Article (original research)
DOI 10.1016/j.fcr.2003.11.001
Open Access Status Not yet assessed
Volume 87
Issue 2-3
Start page 235
End page 244
Total pages 10
Editor P.H. Graham
J.M. Lenne
D. Chatel
Place of publication Netherlands
Publisher Elsevier
Language eng
Subject C1
300201 Plant Biochemistry and Physiology
620107 Cotton
Abstract Bacillus thuringiensis (Bt) transgenic cotton has shown changes of vegetative and reproductive growth characteristics. The objective of this study was to investigate the physiological change of nitrogen metabolism that related closely to the growth in Bt cotton cultivars. The study Was undertaken on two 131 transgenic cotton cultivars and their parents, one conventional (Xingyang822) and recurrent parent (Sumian No. 9), the other a hybrid (Kumian No. 1) and female parent (Yumian No. 1), during the 2001 and 2002 growing seasons at the Yangzhou University Farm, Yangzhou, China. In the 2001 study, The results indicated that the Bt cotton cultivars were higher than their parents in leaf total nitrogen, free amino acid and soluble protein content, greater in NR and GPT activity, and lower in protease activity, during peak square and boll developing period. The biggest increase of total nitrogen was at peak boll period, which increased by 36.01 and 18.96% for Kumian No. I and Xingyang822, respectively. There were similar results for free amino acid and soluble protein content. The results showed further in 2002 study that NR activity increased dramatically at peak square and early boll open period, the biggest increase at early boll open period, with Kumian No. I and Xingyan,822 being 87.5 and 61.4% higher than their parent, respectively, the biggest increase of GPT activity was at peak boll period, with Kumian No. I and Xingyang822 being 39.1 and 29.1% higher than their parent, respectively. However, protease activity of Bt cultivars reduced significantly before flowering and early boll open period, the biggest decrease was before flowering period, with Kumian No. I being more than 30%, Xingyang822 being 26.5% at peak square period. Moreover, the boll total nitrogen content reduced sharply. The results suggest that the Bt cotton cultivars have higher intensity of leaf nitrogen metabolism than their parent, especially during square and boll development period. It is disadvantage for square development and earlier boll maturity under high nitrogen condition. The cultural practice should aim at reducing leaf nitrogen metabolic strength and keep the balance of vegetative and reproductive growth. (C) 2003 Elsevier B.V. All rights reserved.
Keyword Agronomy
Bt Cottons
Nitrogen Metabolism
Nitrate Reductase
Glutamic-pyruvic Transaminase
Protease
Carbon-assimilating Enzymes
Nodule Nitrogen
Alfalfa
Fertilization
Crop
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2005 Higher Education Research Data Collection
School of Agriculture and Food Sciences
 
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Created: Wed, 15 Aug 2007, 14:07:30 EST