Arbuscular mycorrhiza facilitates the accumulation of glycyrrhizin and liquiritin in Glycyrrhiza uralensis under drought stress

Xie, Wei, Hao, Zhipeng, Zhou, Xiaofu, Jiang, Xuelian, Xu, Lijiao, Wu, Songlin, Zhao, Aihua, Zhang, Xin and Chen, Baodong (2018) Arbuscular mycorrhiza facilitates the accumulation of glycyrrhizin and liquiritin in Glycyrrhiza uralensis under drought stress. Mycorrhiza, 28 3: 285-300. doi:10.1007/s00572-018-0827-y


Author Xie, Wei
Hao, Zhipeng
Zhou, Xiaofu
Jiang, Xuelian
Xu, Lijiao
Wu, Songlin
Zhao, Aihua
Zhang, Xin
Chen, Baodong
Title Arbuscular mycorrhiza facilitates the accumulation of glycyrrhizin and liquiritin in Glycyrrhiza uralensis under drought stress
Journal name Mycorrhiza   Check publisher's open access policy
ISSN 1432-1890
0940-6360
Publication date 2018-02-17
Year available 2018
Sub-type Article (original research)
DOI 10.1007/s00572-018-0827-y
Open Access Status Not yet assessed
Volume 28
Issue 3
Start page 285
End page 300
Total pages 16
Place of publication Heidelberg, Germany
Publisher Springer
Language eng
Abstract Liquorice (Glycyrrhiza uralensis) is an important medicinal plant for which there is a huge market demand. It has been reported that arbuscular mycorrhizal (AM) symbiosis and drought stress can stimulate the accumulation of the active ingredients, glycyrrhizin and liquiritin, in liquorice plants, but the potential interactions of AM symbiosis and drought stress remain largely unknown. In the present work, we investigated mycorrhizal effects on plant growth and accumulation of glycyrrhizin and liquiritin in liquorice plants under different water regimes. The results indicated that AM plants generally exhibited better growth and physiological status including stomatal conductance, photosynthesis rate, and water use efficiency compared with non-AM plants. AM inoculation up-regulated the expression of an aquaporin gene PIP and decreased root abscisic acid (ABA) concentrations under drought stress. In general, AM plants displayed lower root carbon (C) and nitrogen (N) concentrations, higher phosphorus (P) concentrations, and therefore, lower C:P and N:P ratios but higher C:N ratio than non-AM plants. On the other hand, AM inoculation increased root glycyrrhizin and liquiritin concentrations, and the mycorrhizal effects were more pronounced under moderate drought stress than under well-watered condition or severe drought stress for glycyrrhizin accumulation. The accumulation of glycyrrhizin and liquiritin in AM plants was consistent with the C:N ratio changes in support of the carbon-nutrient balance hypothesis. Moreover, the glycyrrhizin accumulation was positively correlated with the expression of glycyrrhizin biosynthesis genes SQS1, β-AS, CYP88D6, and CYP72A154. By contrast, no significant interaction of AM inoculation with water treatment was observed for liquiritin accumulation, while we similarly observed a positive correlation between liquiritin accumulation and the expression of a liquiritin biosynthesis gene CHS. These results suggested that AM inoculation in combination with proper water management potentially could improve glycyrrhizin and liquiritin accumulation in liquorice roots and may be practiced to promote liquorice cultivation.
Keyword Drought
Gene expression
Liquorice
Medicinal plants
Mycorrhizal symbiosis
Secondary metabolite
Q-Index Code C1
Q-Index Status Provisional Code
Grant ID 41571250
41371264
2016YFC0500702
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
Sustainable Minerals Institute Publications
 
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Created: Wed, 21 Feb 2018, 11:01:47 EST