Crim1 is required for maintenance of the ocular lens epithelium

Tam, Oliver H., Pennisi, David, Wilkinson, Lorine, Little, Melissa H., Wazin, Fatima, Wan, Victor L. and Lovicu, Frank J. (2018) Crim1 is required for maintenance of the ocular lens epithelium. Experimental Eye Research, 170 58-66. doi:10.1016/j.exer.2018.02.012

Author Tam, Oliver H.
Pennisi, David
Wilkinson, Lorine
Little, Melissa H.
Wazin, Fatima
Wan, Victor L.
Lovicu, Frank J.
Title Crim1 is required for maintenance of the ocular lens epithelium
Journal name Experimental Eye Research   Check publisher's open access policy
ISSN 1096-0007
Publication date 2018-02-16
Year available 2018
Sub-type Article (original research)
DOI 10.1016/j.exer.2018.02.012
Open Access Status Not yet assessed
Volume 170
Start page 58
End page 66
Total pages 9
Place of publication London, United Kingdom
Publisher Academic Press
Abstract The development and growth of the vertebrate ocular lens is dependent on the regulated proliferation of an anterior monolayer of epithelial cells, and their subsequent differentiation into elongate fiber cells. The growth factor rich ocular media that bathes the lens mediates these cellular processes, and their respective intracellular signaling pathways are in turn regulated to ensure that the proper lens architecture is maintained. Recent studies have proposed that Cysteine Rich Motor Neuron 1 (Crim1), a transmembrane protein involved in organogenesis of many tissues, might influence cell adhesion, polarity and proliferation in the lens by regulating integrin-signaling. Here, we characterise the lens and eyes of the Crim1mutant mice, and show that the loss of Crim1 function in the ocular tissues results in inappropriate differentiation of the lens epithelium into fiber cells. Furthermore, restoration of Crim1 levels in just the lens tissue of Crim1mice is sufficient to ameliorate most of the dysgenesis observed in the mutant animals. Based on our findings, we propose that tight regulation of Crim1 activity is required for maintenance of the lens epithelium, and its depletion leads to ectopic differentiation into fiber cells, dramatically altering lens structure and ultimately leading to microphthalmia and aphakia.
Keyword Crim1
Fiber differentiation
Lens epithelium
Lens fibers
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
Institute for Molecular Bioscience - Publications
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Created: Wed, 21 Feb 2018, 11:00:57 EST