Characterization of Rab23, a negative regulator of sonic hedgehog signaling

Evans, Timothy M., Simpson, Fiona, Parton, Robert G. and Wicking, Carol (2005). Characterization of Rab23, a negative regulator of sonic hedgehog signaling. In William E. Balch, Channing J. Der and Alan Hall (Ed.), GTPases regulating membrane targeting and fusion (pp. 759-777) Maryland Heights, MO, United States: Academic Press. doi:10.1016/S0076-6879(05)03066-1


Author Evans, Timothy M.
Simpson, Fiona
Parton, Robert G.
Wicking, Carol
Title of chapter Characterization of Rab23, a negative regulator of sonic hedgehog signaling
Title of book GTPases regulating membrane targeting and fusion
Place of Publication Maryland Heights, MO, United States
Publisher Academic Press
Publication Year 2005
Sub-type Research book chapter (original research)
DOI 10.1016/S0076-6879(05)03066-1
Series Methods in Enzymology
ISBN 9780121828080; 0076-6879
ISSN 0076-6879
Editor William E. Balch
Channing J. Der
Alan Hall
Volume number 403
Chapter number 66
Start page 759
End page 777
Total pages 19
Total chapters 69
Language eng
Subjects 270104 Membrane Biology
780105 Biological sciences
B1
Formatted Abstract/Summary
The hedgehog signaling pathway is indispensable in embryogenesis, being responsible for the development of a wide array of vertebrate organs. Given its importance in embryogenesis, the precise regulation of hedgehog signaling is crucial. Aberrant activation of this pathway in postnatal life has been associated with a number of tumor types, reinforcing the role of developmental signaling pathways in tumorigenesis. The small GTPase Rab23 acts as a negative regulator of the hedgehog signaling pathway, most notably in the vertebrate neural system. By analogy with studies of other Rab proteins, analysis of the localization of wild‐type and constitutively active and inactive forms of Rab23 provides the potential to shed light on the role of Rab23 at the cellular level. We previously produced expression constructs encoding these proteins for analysis in mammalian cell cultures at both the light and the electron microscopy level. This revealed that both wild‐type and active Rab23 localizes to the plasma membrane and to endocytic vesicles (T. M. Evans et al. [2003] Traffic 4, 869–884). We describe the methods used to design and make the Rab23 expression constructs, and to assess their localization relative to key hedgehog pathways and endocytic markers in both transiently and stably transfected cell cultures. 
Q-Index Code B1
Q-Index Status Provisional Code
Institutional Status UQ

 
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Created: Tue, 14 Aug 2007, 22:22:47 EST