Calcium-dependent protein kinases in cotton: insights into early plant responses to salt stress

Gao, Wei, Xu, Fu-Chun, Guo, Dan-Dan, Zhao, Jing-Ruo, Liu, Ji, Guo, Ya-Wei, Singh, Prashant Kumar, Ma, Xiao-Nan, Long, Lu, Botella, Jose Ramon and Song, Chun-Peng (2018) Calcium-dependent protein kinases in cotton: insights into early plant responses to salt stress. BMC Plant Biology, 18 1: 1-15. doi:10.1186/s12870-018-1230-8


Author Gao, Wei
Xu, Fu-Chun
Guo, Dan-Dan
Zhao, Jing-Ruo
Liu, Ji
Guo, Ya-Wei
Singh, Prashant Kumar
Ma, Xiao-Nan
Long, Lu
Botella, Jose Ramon
Song, Chun-Peng
Title Calcium-dependent protein kinases in cotton: insights into early plant responses to salt stress
Journal name BMC Plant Biology   Check publisher's open access policy
ISSN 1471-2229
Publication date 2018-01-17
Year available 2018
Sub-type Article (original research)
DOI 10.1186/s12870-018-1230-8
Open Access Status DOI
Volume 18
Issue 1
Start page 1
End page 15
Total pages 15
Place of publication London, United Kingdom
Publisher BioMed Central
Language eng
Subject 1110 Plant Science
Abstract Soil salinization is one of the major environmental constraints to plant growth and agricultural production worldwide. Signaling components involving calcium (Ca2+) and the downstream calcium-dependent protein kinases (CPKs) play key roles in the perception and transduction of stress signals. However, the study of CPKs in cotton and their functions in response to salt stress remain unexplored.

A total of 98 predicted CPKs were identified from upland cotton (Gossypium hirsutum L. 'TM-1'), and phylogenetic analyses classified them into four groups. Gene family distribution studies have revealed the substantial impacts of the genome duplication events to the total number of GhCPKs. Transcriptome analyses showed a wide distribution of CPKs' expression among different organs. A total of 19 CPKs were selected for their rapid responses to salt stress at the transcriptional level, most of which were also incduced by the thylene-releasing chemical ethephon, suggesting a partal overlap of the salinity and ethylene responses. Silencing of 4 of the 19 CPKs (GhCPK8, GhCPK38, GhCPK54, and GhCPK55) severely compromised the basal cotton resistance to salt stress.

Our genome-wide expression analysis of CPK genes from up-land cotton suggests that CPKs are involved in multiple developmental responses as well as the response to different abiotic stresses. A cluster of the cotton CPKs was shown to participate in the early signaling events in cotton responses to salt stress. Our results provide significant insights on functional analysis of CPKs in cotton, especially in the context of cotton adaptions to salt stress.
Keyword Calcium dependent protein kinase
Genome-wide identification
Gossypium hirsutum
Transcriptome
Virus-induced gene silencing
Q-Index Code C1
Q-Index Status Provisional Code
Grant ID 31601344
2016ZX08009-003
2016YFD0101006
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
School of Agriculture and Food Sciences
 
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Created: Wed, 24 Jan 2018, 12:13:41 EST