Disruption to the 5-HT7 Receptor Following Hypoxia-Ischemia in the Immature Rodent Brain

Wixey, Julie A, Reinebrant, Hanna E, Chand, Kirat K and Buller, Kathryn M (2018) Disruption to the 5-HT7 Receptor Following Hypoxia-Ischemia in the Immature Rodent Brain. Neurochemical research, 43 3: 711-720. doi:10.1007/s11064-018-2473-3

Author Wixey, Julie A
Reinebrant, Hanna E
Chand, Kirat K
Buller, Kathryn M
Title Disruption to the 5-HT7 Receptor Following Hypoxia-Ischemia in the Immature Rodent Brain
Journal name Neurochemical research   Check publisher's open access policy
ISSN 1573-6903
Publication date 2018-01-22
Year available 2018
Sub-type Article (original research)
DOI 10.1007/s11064-018-2473-3
Open Access Status Not yet assessed
Volume 43
Issue 3
Start page 711
End page 720
Total pages 10
Place of publication New York, NY United States
Publisher Springer
Language eng
Subject 1303 Biochemistry
2804 Cellular and Molecular Neuroscience
Abstract It has become increasingly evident the serotonergic (5-hydroxytryptamine, 5-HT) system is an important central neuronal network disrupted following neonatal hypoxic-ischemic (HI) insults. Serotonin acts via a variety of receptor subtypes that are differentially associated with behavioural and cognitive mechanisms. The 5-HT7 receptor is purported to play a key role in epilepsy, anxiety, learning and memory and neuropsychiatric disorders. Furthermore, the 5-HT7 receptor is highly localized in brain regions damaged following neonatal HI insults. Utilising our well-established neonatal HI model in the postnatal day 3 (P3) rat pup we demonstrated a significant decrease in levels of the 5-HT7 protein in the frontal cortex, thalamus and brainstem one week after insult. We also observed a relative decrease in both the cytosolic and membrane fractions of 5-HT7. The 5-HT7 receptor was detected on neurons throughout the cortex and thalamus, and 5-HT cell bodies in the brainstem. However we found no evidence of 5-HT7 co-localisation on microglia or astrocytes. Moreover, minocycline treatment did not significantly prevent the HI-induced reductions in 5-HT7. In conclusion, neonatal HI injury caused significant disruption to 5-HT7 receptors in the forebrain and brainstem. Yet the use of minocycline to inhibit activated microglia, did not prevent the HI-induced changes in 5-HT7 expression.
Keyword Astrocytes
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Mater Research Institute-UQ (MRI-UQ)
Faculty of Medicine
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Citation counts: Scopus Citation Count Cited 0 times in Scopus Article
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Created: Wed, 24 Jan 2018, 12:01:19 EST