An ex vivo model to quantitatively analyze cell migration in tissue

O'Leary, Conor J., Weston, Mikail and McDermott, Kieran W. (2017) An ex vivo model to quantitatively analyze cell migration in tissue. Developmental Dynamics, 247 1: 201-211. doi:10.1002/dvdy.24562

Author O'Leary, Conor J.
Weston, Mikail
McDermott, Kieran W.
Title An ex vivo model to quantitatively analyze cell migration in tissue
Journal name Developmental Dynamics   Check publisher's open access policy
ISSN 1097-0177
Publication date 2017-09-18
Year available 2017
Sub-type Article (original research)
DOI 10.1002/dvdy.24562
Open Access Status Not yet assessed
Volume 247
Issue 1
Start page 201
End page 211
Total pages 11
Place of publication Hoboken, NJ United States
Publisher John Wiley and Sons
Language eng
Subject 1309 Developmental Biology
Abstract Background: Within the developing central nervous system, the ability of cells to migrate throughout the tissue parenchyma to reach their target destination and undergo terminal differentiation is vital to normal central nervous system (CNS) development. To develop novel therapies to treat the injured CNS, it is essential that the migratory behavior of cell populations is understood. Many studies have examined the ability of individual neurons to migrate through the developing CNS, describing specific modes of migration including locomotion and somal translocation. Few studies have investigated the mass migration of large populations of neural progenitors, particularly in the developing the spinal cord. Here, we describe a method to robustly analyze large numbers of migrating cells using a co-culture assay. Results: The ex vivo tissue model promotes the survival and differentiation of co-cultured progenitor cells. Using this assay, we demonstrate that migrating neuroepithelial progenitor cells display region specific migration patterns within the dorsal and ventral spinal cord at defined developmental time points. Conclusions: The technique described here is a viable ex vivo model to quantitatively analyze cell migration and differentiation. We demonstrate the ability to detect changes in cell migration within distinct tissue region across tissue samples using the technique described here. Developmental Dynamics 247:201–211, 2018.
Keyword Cartesian coordinate analysis
cell migration
developing spinal cord
radial cell distance
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
School of Biomedical Sciences Publications
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Created: Sun, 31 Dec 2017, 21:37:12 EST