In vivo recordings at the Caenorhabditis elegans neuromuscular junction

Gao, Shangbang and Hu, Zhitao (2018). In vivo recordings at the Caenorhabditis elegans neuromuscular junction. In Extracellular Recording Approaches (pp. 143-156) New York, NY United States: Humana Press. doi:10.1007/978-1-4939-7549-5_8

Author Gao, Shangbang
Hu, Zhitao
Title of chapter In vivo recordings at the Caenorhabditis elegans neuromuscular junction
Title of book Extracellular Recording Approaches
Place of Publication New York, NY United States
Publisher Humana Press
Publication Year 2018
Sub-type Research book chapter (original research)
DOI 10.1007/978-1-4939-7549-5_8
Open Access Status Not yet assessed
Series Neuromethods
ISBN 9781493975488
ISSN 1940-6045
Volume number 134
Chapter number 8
Start page 143
End page 156
Total pages 14
Total chapters 14
Language eng
Abstract/Summary The communication between neurons occurs via synapses, specialized connections with other cells or tissues (e.g., muscles). The function of the synapse is mainly determined by synaptic vesicle exocytosis from the presynaptic nerve terminal, which contains hundreds of vesicles that are filled with neurotransmitters. Electrophysiology provides a direct way to measure synaptic vesicle release and investigate synaptic properties. C. elegans neuromuscular junction (NMJ) has been established as a model system to study synaptic function because of the application of electrophysiological recording. Here we describe a recently developed patch-clamp recording technique in neuromuscular junction of C. elegans including details of the construction of typical electrophysiology rig, the proper dissection technique, and the recording of different types of synaptic currents.
Keyword C. elegans
Neuromuscular junction
Patch clamp
Synaptic vesicle
Q-Index Code B1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Book Chapter
Collections: HERDC Pre-Audit
Queensland Brain Institute Publications
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Created: Sun, 31 Dec 2017, 21:36:47 EST