Oncogenic transformation of lung cells results in distinct exosome protein profile similar to the cell of origin

Lobb, Richard J., Hastie, Marcus L., Norris, Emma L., van Amerongen, Rosa, Gorman, Jeffrey J. and Möller, Andreas (2017) Oncogenic transformation of lung cells results in distinct exosome protein profile similar to the cell of origin. Proteomics, 17 23-24: 1-10. doi:10.1002/pmic.201600432


Author Lobb, Richard J.
Hastie, Marcus L.
Norris, Emma L.
van Amerongen, Rosa
Gorman, Jeffrey J.
Möller, Andreas
Title Oncogenic transformation of lung cells results in distinct exosome protein profile similar to the cell of origin
Journal name Proteomics   Check publisher's open access policy
ISSN 1615-9861
1615-9853
Publication date 2017-09-15
Year available 2017
Sub-type Article (original research)
DOI 10.1002/pmic.201600432
Open Access Status Not yet assessed
Volume 17
Issue 23-24
Start page 1
End page 10
Total pages 10
Place of publication Weinheim, Germany
Publisher Wiley-VCH Verlag
Language eng
Subject 1303 Biochemistry
1312 Molecular Biology
Abstract Lung cancer is responsible for the highest rate of cancer mortality worldwide. Lung cancer patients are often ineligible for tumor biopsies due to comorbidities. As a result, patients may not have the most effective treatment regimens administered. Patients with mutations in the epidermal growth factor receptor (EGFR) have improved survival in response to EGFR tyrosine kinase inhibitors. A noninvasive method of determining EGFR mutations in patients would have promising clinical applications. Exosomes have the potential to be noninvasive novel diagnostic markers in cancer. Using MS analysis, we identify differentially abundant cell and exosome proteins induced by mutations in p53 and EGFR in lung cells. Importantly, mutations in p53 and EGFR alter cell and exosome protein content compared to an isogenic normal lung epithelial cell. For some proteins, mutation had similar effects in the cell of origin and exosomes. Differences between the cells of origin and exosomes were also apparent, which may reflect specific packaging of proteins into exosomes. These findings that mutations alter protein abundance in exosomes suggest that analysis of exosomes may be beneficial in the diagnosis of oncogenic mutations.
Keyword diagnostic
EGFR
exosomes
non-small cell lung cancer
Q-Index Code C1
Q-Index Status Provisional Code
Grant ID APP1068510
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
School of Biomedical Sciences Publications
School of Medicine Publications
 
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Created: Sun, 31 Dec 2017, 16:17:26 EST