Fetal Bone Marrow-Derived Mesenchymal Stem/Stromal Cells Enhance Humanization and Bone Formation of BMP7 Loaded Scaffolds

Shafiee, Abbas, Baldwin, Jeremy G., Patel, Jatin, Holzapfel, Boris M., Fisk, Nicholas M., Khosrotehrani, Kiarash and Hutmacher, Dietmar W. (2017) Fetal Bone Marrow-Derived Mesenchymal Stem/Stromal Cells Enhance Humanization and Bone Formation of BMP7 Loaded Scaffolds. Biotechnology Journal, 12 12: . doi:10.1002/biot.201700414


Author Shafiee, Abbas
Baldwin, Jeremy G.
Patel, Jatin
Holzapfel, Boris M.
Fisk, Nicholas M.
Khosrotehrani, Kiarash
Hutmacher, Dietmar W.
Title Fetal Bone Marrow-Derived Mesenchymal Stem/Stromal Cells Enhance Humanization and Bone Formation of BMP7 Loaded Scaffolds
Journal name Biotechnology Journal   Check publisher's open access policy
ISSN 1860-6768
1860-7314
Publication date 2017-09-25
Year available 2017
Sub-type Article (original research)
DOI 10.1002/biot.201700414
Open Access Status Not yet assessed
Volume 12
Issue 12
Total pages 7
Place of publication Weinheim, Germany
Publisher WILEY
Language eng
Subject 2402 Applied Microbiology and Biotechnology
1313 Molecular Medicine
Abstract Tissue engineered constructs built with human cells capable of generating a bone-like organ within the mouse have attracted considerable interest over the past decade. Here, we aimed to compare the utility of human mesenchymal stem/stromal cells (MSC) isolated from fetal term placenta (fPL-MSC) and fetal first trimester bone marrow (fBM-MSC) in a polycaprolactone scaffold/BMP7-based model in nude mice. Furthermore, fPL-MSC were co-seeded with fetal placenta-derived endothelial colony forming cells (ECFC) to assess the impact of ECFC on fPL-MSC osteogenesis. X-ray radiography and micro computed tomography analyses showed enhanced bone formation in all BMP7 groups; however there was no difference after 2 months in bone formation between scaffolds seeded with fPL-MSC alone or combination of ECFC and fPL-MSC. Of interest, fBM-MSC showed the highest level of bone formation. Additionally, endochondral ossification contributed in generation of bone in fBM-MSC. Histological analysis showed the primary role of BMP in generation of cortical and trabecular bone, and the recruitment of hematopoietic cells to the scaffolds. Current in vivo engineered bone organs can potentially be used for drug screening or as models to study bone tissue development in combination with haematopoiesis.
Keyword Colony-Forming Cells
Human Term Placenta
Endochondral Ossification
Stem-Cells
Organ
Revascularization
Lymphocytes
Engraftment
Liver
Msc
Q-Index Code C1
Q-Index Status Provisional Code
Grant ID 1082313
DFG HO 5068/1-1
NBCF IN-15-047
WWCR 15-11563
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: UQ Centre for Clinical Research Publications
HERDC Pre-Audit
UQ Diamantina Institute Publications
 
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Created: Sat, 30 Dec 2017, 23:02:32 EST