Clinical veterinary proteomics: Techniques and approaches to decipher the animal plasma proteome

Ghodasara, P., Sadowski, P., Satake, N., Kopp, S. and Mills, P. C. (2017) Clinical veterinary proteomics: Techniques and approaches to decipher the animal plasma proteome. Veterinary Journal, 230 6-12. doi:10.1016/j.tvjl.2017.10.022


Author Ghodasara, P.
Sadowski, P.
Satake, N.
Kopp, S.
Mills, P. C.
Title Clinical veterinary proteomics: Techniques and approaches to decipher the animal plasma proteome
Journal name Veterinary Journal   Check publisher's open access policy
ISSN 1532-2971
1090-0233
Publication date 2017-11-11
Year available 2017
Sub-type Critical review of research, literature review, critical commentary
DOI 10.1016/j.tvjl.2017.10.022
Open Access Status Not yet assessed
Volume 230
Start page 6
End page 12
Total pages 7
Place of publication London, United Kingdom
Publisher Elsevier
Language eng
Subject 1103 Animal Science and Zoology
3400 Veterinary
Abstract Over the last two decades, technological advancements in the field of proteomics have advanced our understanding of the complex biological systems of living organisms. Techniques based on mass spectrometry (MS) have emerged as powerful tools to contextualise existing genomic information and to create quantitative protein profiles from plasma, tissues or cell lines of various species. Proteomic approaches have been used increasingly in veterinary science to investigate biological processes responsible for growth, reproduction and pathological events. However, the adoption of proteomic approaches by veterinary investigators lags behind that of researchers in the human medical field. Furthermore, in contrast to human proteomics studies, interpretation of veterinary proteomic data is difficult due to the limited protein databases available for many animal species. This review article examines the current use of advanced proteomics techniques for evaluation of animal health and welfare and covers the current status of clinical veterinary proteomics research, including successful protein identification and data interpretation studies. It includes a description of an emerging tool, sequential window acquisition of all theoretical fragment ion mass spectra (SWATH-MS), available on selected mass spectrometry instruments. This newly developed data acquisition technique combines advantages of discovery and targeted proteomics approaches, and thus has the potential to advance the veterinary proteomics field by enhancing identification and reproducibility of proteomics data.
Keyword Data interpretation
Mass spectrometry
Protein profile
Sequential window acquisition of all theoretical fragment ion mass spectra (SWATH-MS)
Veterinary proteomics
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Critical review of research, literature review, critical commentary
Collections: HERDC Pre-Audit
School of Veterinary Science Publications
 
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Created: Sun, 24 Dec 2017, 01:03:20 EST