Genome Editing—Principles and Applications for Functional Genomics Research and Crop Improvement

Zhang, Hui, Zhang, Jinshan, Lang, Zhaobo, Botella, José Ramón and Zhu, Jian-Kang (2017) Genome Editing—Principles and Applications for Functional Genomics Research and Crop Improvement. Critical Reviews in Plant Sciences, 36 4: 291-309. doi:10.1080/07352689.2017.1402989


Author Zhang, Hui
Zhang, Jinshan
Lang, Zhaobo
Botella, José Ramón
Zhu, Jian-Kang
Title Genome Editing—Principles and Applications for Functional Genomics Research and Crop Improvement
Journal name Critical Reviews in Plant Sciences   Check publisher's open access policy
ISSN 1549-7836
0735-2689
Publication date 2017-12-11
Year available 2017
Sub-type Article (original research)
DOI 10.1080/07352689.2017.1402989
Open Access Status DOI
Volume 36
Issue 4
Start page 291
End page 309
Total pages 19
Place of publication New York, United States
Publisher Taylor and Francis
Language eng
Subject 1110 Plant Science
Abstract Genome editing technologies are powerful tools for studying gene function and for crop improvement. The technologies rely on engineered endonucleases to generate double stranded breaks (DSBs) at target loci. The DSBs are repaired through the error-prone non-homologous end joining (NHEJ) and homology-directed repair (HDR) pathways in cells, resulting in mutations and sequence replacement, respectively. In the widely used CRISPR/Cas9 system, the endonuclease Cas9 is targeted by a CRISPR small RNA to DNA sequence of interest. In this review, we describe the four available types of genome editing tools, ZFN, TALEN, CRISPR/Cas9 and CRISPR/Cpf1, and show their applications in functional genomics research and precision molecular breeding of crops.
Keyword Cas9
Cpf1
CRISPR
Gene editing
Genomics
Precision molecular breeding
TALEN
ZFN
Q-Index Code C1
Q-Index Status Provisional Code
Grant ID 20140029
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
School of Agriculture and Food Sciences
 
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Created: Sun, 24 Dec 2017, 00:38:24 EST