Cryo-electron microscopy of vitreous sections

Al-Amoudi, Ashraf, Chang, Jiin-Ju, Leforestier, Amélie, McDowall, Alasdair, Salamin, Laurée Michel, Norlén, Lars P. O., Richter, Karsten, Blanc, Nathalie Sartori, Studer, Daniel and Dubochet, Jacques (2004) Cryo-electron microscopy of vitreous sections. EMBO Journal, 23 18: 3583-3588. doi:10.1038/sj.emboj.7600366


Author Al-Amoudi, Ashraf
Chang, Jiin-Ju
Leforestier, Amélie
McDowall, Alasdair
Salamin, Laurée Michel
Norlén, Lars P. O.
Richter, Karsten
Blanc, Nathalie Sartori
Studer, Daniel
Dubochet, Jacques
Title Cryo-electron microscopy of vitreous sections
Journal name EMBO Journal   Check publisher's open access policy
ISSN 0261-4189
Publication date 2004-09-15
Sub-type Critical review of research, literature review, critical commentary
DOI 10.1038/sj.emboj.7600366
Open Access Status Not yet assessed
Volume 23
Issue 18
Start page 3583
End page 3588
Total pages 6
Language eng
Subject 2800 Neuroscience
1312 Molecular Biology
1300 Biochemistry, Genetics and Molecular Biology
2400 Immunology and Microbiology
Abstract Since the beginning of the 1980s, cryo-electron microscopy of a thin film of vitrified aqueous suspension has made it possible to observe biological particles in their native state, in the absence of the usual artefacts of dehydration and staining. Combined with 3-d reconstruction, it has become an important tool for structural molecular biology. Larger objects such as cells and tissues cannot generally be squeezed in a thin enough film. Cryo-electron microscopy of vitreous sections (CEMOVIS) provides then a solution. It requires vitrification of a sizable piece of biological material and cutting it into ultrathin sections, which are observed in the vitrified state. Each of these operations raises serious difficulties that have now been overcome. In general, the native state seen with CEMOVIS is very different from what has been seen before and it is seen in more detail. CEMOVIS will give its full potential when combined with computerized electron tomography for 3-d reconstruction.
Keyword Bacterial envelope
Desmosome
High-pressure freezing
Intermediate filament
Vitrification
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Critical review of research, literature review, critical commentary
Collection: Scopus Import - Archived
 
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Created: Fri, 22 Dec 2017, 13:19:02 EST