Investigating methodological differences in the assessment of dendritic morphology of basolateral amygdala principal neurons – a comparison of Golgi-Cox and neurobiotin electroporation techniques

Klenowski, Paul M., Wright, Sophie E., Mu, Erica W.H., Noakes, Peter G., Lavidis, Nickolas A., Bartlett, Selena E., Bellingham, Mark C. and Fogarty, Matthew J. (2017) Investigating methodological differences in the assessment of dendritic morphology of basolateral amygdala principal neurons – a comparison of Golgi-Cox and neurobiotin electroporation techniques. Brain Sciences, 7 12: 1-12. doi:10.3390/brainsci7120165


Author Klenowski, Paul M.
Wright, Sophie E.
Mu, Erica W.H.
Noakes, Peter G.
Lavidis, Nickolas A.
Bartlett, Selena E.
Bellingham, Mark C.
Fogarty, Matthew J.
Title Investigating methodological differences in the assessment of dendritic morphology of basolateral amygdala principal neurons – a comparison of Golgi-Cox and neurobiotin electroporation techniques
Journal name Brain Sciences   Check publisher's open access policy
ISSN 2076-3425
Publication date 2017-12-19
Year available 2017
Sub-type Article (original research)
DOI 10.3390/brainsci7120165
Open Access Status DOI
Volume 7
Issue 12
Start page 1
End page 12
Total pages 12
Place of publication Basel, Switzerland
Publisher M D P I AG
Language eng
Abstract Quantitative assessments of neuronal subtypes in numerous brain regions show large variations in dendritic arbor size. A critical experimental factor is the method used to visualize neurons. We chose to investigate quantitative differences in basolateral amygdala (BLA) principal neuron morphology using two of the most common visualization methods: Golgi-Cox staining and neurobiotin (NB) filling. We show in 8-week-old Wistar rats that NB-filling reveals significantly larger dendritic arbors and different spine densities, compared to Golgi-Cox-stained BLA neurons. Our results demonstrate important differences and provide methodological insights into quantitative disparities of BLA principal neuron morphology reported in the literature.
Keyword Golgi–Cox
basolateral amygdala
dendrites
neurobiotin
principal neuron
spines
Q-Index Code C1
Q-Index Status Provisional Code
Grant ID APP1065884
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
Queensland Brain Institute Publications
School of Biomedical Sciences Publications
 
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Created: Wed, 20 Dec 2017, 12:12:18 EST by Dr Mark Bellingham on behalf of School of Biomedical Sciences