Advanced DNA-Based Point-of-Care Diagnostic Methods for Plant Diseases Detection

Lau, Han Yih and Botella, Jose R. (2017) Advanced DNA-Based Point-of-Care Diagnostic Methods for Plant Diseases Detection. Frontiers in Plant Science, 8 2016. doi:10.3389/fpls.2017.02016


Author Lau, Han Yih
Botella, Jose R.
Title Advanced DNA-Based Point-of-Care Diagnostic Methods for Plant Diseases Detection
Journal name Frontiers in Plant Science   Check publisher's open access policy
ISSN 1664-462X
Publication date 2017-12-06
Year available 2017
Sub-type Critical review of research, literature review, critical commentary
DOI 10.3389/fpls.2017.02016
Open Access Status DOI
Volume 8
Start page 2016
Total pages 14
Place of publication Lausanne, Switzerland
Publisher Frontiers Research Foundation
Language eng
Subject 1110 Plant Science
Abstract Diagnostic technologies for the detection of plant pathogens with point-of-care capability and high multiplexing ability are an essential tool in the fight to reduce the large agricultural production losses caused by plant diseases. The main desirable characteristics for such diagnostic assays are high specificity, sensitivity, reproducibility, quickness, cost efficiency and high-throughput multiplex detection capability. This article describes and discusses various DNA-based point-of care diagnostic methods for applications in plant disease detection. Polymerase chain reaction (PCR) is the most common DNA amplification technology used for detecting various plant and animal pathogens. However, subsequent to PCR based assays, several types of nucleic acid amplification technologies have been developed to achieve higher sensitivity, rapid detection as well as suitable for field applications such as loop-mediated isothermal amplification, helicase-dependent amplification, rolling circle amplification, recombinase polymerase amplification, and molecular inversion probe. The principle behind these technologies has been thoroughly discussed in several review papers; herein we emphasize the application of these technologies to detect plant pathogens by outlining the advantages and disadvantages of each technology in detail.
Keyword Mediated Isothermal Amplification
Rolling-Circle Amplification
Helicase-Dependent Amplification
Recombinase Polymerase Amplification
Linked-Immunosorbent-Assay
Molecular Inversion Probes
Real-Time Pcr
Droplet Digital Pcr
Plum-Pox-Virus
Rapid Detection
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Critical review of research, literature review, critical commentary
Collections: HERDC Pre-Audit
School of Agriculture and Food Sciences
 
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Created: Sat, 16 Dec 2017, 23:07:28 EST