An immunoassay for human D dimer using monoclonal antibodies

Rylatt, D. B., Blake, A. S., Cottis, L. E., Massingham, D. A., Fletcher, W. A., Masci, P. P., Whitaker, A. N., Elms, M., Bunce, I., Webber, A. J., Wyatt, D. and Bundesen, P. G. (1983) An immunoassay for human D dimer using monoclonal antibodies. Thrombosis Research, 31 6: 767-778. doi:10.1016/0049-3848(83)90108-1


Author Rylatt, D. B.
Blake, A. S.
Cottis, L. E.
Massingham, D. A.
Fletcher, W. A.
Masci, P. P.
Whitaker, A. N.
Elms, M.
Bunce, I.
Webber, A. J.
Wyatt, D.
Bundesen, P. G.
Title An immunoassay for human D dimer using monoclonal antibodies
Journal name Thrombosis Research   Check publisher's open access policy
ISSN 0049-3848
Publication date 1983-09-15
Sub-type Article (original research)
DOI 10.1016/0049-3848(83)90108-1
Open Access Status Not yet assessed
Volume 31
Issue 6
Start page 767
End page 778
Total pages 12
Language eng
Subject 2720 Hematology
Abstract Monoclonal antibodies (MAb) were raised against human D dimer. The hybridomas were screened with a solid phase enzyme immunoassay against D dimer and fibrinogen degradation products. Among the panel of MAb identified, two distinct patterns emerged; the majority belonging to a panspecific class reacting against epitopes present on both D dimer and fibrinogen degradation product Dcate and a monospecific class reacting with determinants apparently present only on D dimer. A number of MAb were further characterised for their ability to specifically capture antigen in a solid phase enzyme immunoassay and assays were developed which have a sensitivity of 10 ng/ml for D dimer or crosslinked fibrin derivatives and may be suitable for detection of crosslinked derivatives in serum and plasma samples in a clinical situation.
Keyword D dimer
enzyme immunoassay
fibrinolysis
intravascular coagulation
Monoclonal antibodies
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
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Created: Fri, 15 Dec 2017, 01:48:13 EST