Investigation of in vitro measurable sperm attributes and their influence on electroejaculated bull semen with a fixed-time artificial insemination protocol in Australian Bos indicus cattle

Satake, N, Edwards, Saa, Tutt, D, McGowan, M R and Boe-Hansen, G B (2017) Investigation of in vitro measurable sperm attributes and their influence on electroejaculated bull semen with a fixed-time artificial insemination protocol in Australian Bos indicus cattle. Reproduction in domestic animals = Zuchthygiene, 53 2: 414-422. doi:10.1111/rda.13121


Author Satake, N
Edwards, Saa
Tutt, D
McGowan, M R
Boe-Hansen, G B
Title Investigation of in vitro measurable sperm attributes and their influence on electroejaculated bull semen with a fixed-time artificial insemination protocol in Australian Bos indicus cattle
Journal name Reproduction in domestic animals = Zuchthygiene   Check publisher's open access policy
ISSN 1439-0531
0936-6768
Publication date 2017-12-11
Year available 2017
Sub-type Article (original research)
DOI 10.1111/rda.13121
Open Access Status Not yet assessed
Volume 53
Issue 2
Start page 414
End page 422
Total pages 9
Place of publication Berlin, Germany
Publisher Wiley-Blackwell
Language eng
Subject 1305 Biotechnology
1103 Animal Science and Zoology
1310 Endocrinology
Abstract Increasing use of fixed-time artificial insemination (FTAI) in beef cattle production has presented an opportunity for the use of fresh or chilled semen as an alternative to standard cryopreserved semen. The objective of this study was to examine in vitro sperm function and pregnancy rate of electroejaculated semen, chilled and stored for 48 hr, compared to conventionally cryopreserved semen with an optimized FTAI protocol in Brahman cattle. Semen from three Brahman bulls was collected, and aliquots were extended in either chilled (at 5°C) or frozen (LN2 ) in a Tris-egg yolk extender base with 2.4% or 7.0% glycerol, respectively. Semen samples were assessed 48 hr after collection or post-thaw and warming, for sperm motility, in vitro sperm function and fertilizing ability, and used in a FTAI programme. The overall pregnancy rates was significantly different (p < .01) after FTAI with frozen (n = 173; 53.2%) and chilled semen (n = 174; 31.6%). In contrast, the in vitro sperm assessment showed that the chilled semen had significantly faster motility (p < .05), a higher proportion of progressively motile spermatozoa (p < .05), with significantly higher proportions of acrosome intact, viable spermatozoa (p < .01). This study showed that reasonable pregnancy rates in Brahman cattle can be achieved using FTAI with chilled semen collected using electroejaculation and stored for up to 48 hr. However, improvements in semen extenders are required in consideration of semen collection method to improve the longevity of sperm fertilizing ability to significantly increase FTAI output using chilled storage of bull semen.
Keyword computer-assisted semen analyser
sperm function
sperm storage
tropically adapted beef cattle
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Veterinary Science Publications
 
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Created: Wed, 13 Dec 2017, 12:03:07 EST