Etr1-1 gene expression alters regeneration patterns in transgenic lettuce stimulating root formation

Kim, J. H. and Botella, J. R. (2004) Etr1-1 gene expression alters regeneration patterns in transgenic lettuce stimulating root formation. Plant Cell Tissue And Organ Culture, 78 1: 69-73. doi:10.1023/B:TICU.0000020396.64257.c1


Author Kim, J. H.
Botella, J. R.
Title Etr1-1 gene expression alters regeneration patterns in transgenic lettuce stimulating root formation
Journal name Plant Cell Tissue And Organ Culture   Check publisher's open access policy
ISSN 0167-6857
Publication date 2004-01-01
Year available 2004
Sub-type Article (original research)
DOI 10.1023/B:TICU.0000020396.64257.c1
Open Access Status Not yet assessed
Volume 78
Issue 1
Start page 69
End page 73
Total pages 5
Place of publication Dordrecht
Publisher Kluwer Academic Publishers
Language eng
Subject C1
270403 Plant Pathology
620208 Vegetables
Abstract We have evaluated the transformation efficiency of two lettuce ( Lactuca sativa L.) cultivars, LE126 and Seagreen, using Agrobacterium tumefaciens- mediated gene transfer. Six- day- old cotyledons were co- cultivated with Agrobacterium cultures carrying binary vectors with two different genetic constructs. The first construct contained the beta- glucuronidase gene ( GUS) under the control of the cauliflower mosaic virus 35S promoter ( CaMV 35S), while the second construct contained the ethylene mutant receptor etr1- 1, which confers ethylene insensitivity, under the control of a leaf senescence- specific promoter ( sag12). Tissues co- cultivated with the GUS construct showed strong regeneration potential with over 90% of explants developing callus masses and 85% of the calli developing shoots. Histochemical GUS assays showed that 85.7% of the plants recovered were transgenic. Very different results were observed when cotyledon explants were co- cultivated with Agrobacteria carrying the etr1- 1 gene. There was a dramatic effect on the regeneration properties of the cultured explants with root formation taking place directly from the cotyledon tissue in 34% of the explants and no callus or shoots observed initially. Eventually callus formed in 10% of cotyledons and some organogenic shoots were obtained ( 2.86%). These results indicate that the ethylene insensitivity conferred by the etr1- 1 gene alters the normal pattern of regeneration in lettuce cotyledons, inhibiting the formation of shoots and stimulating root formation during regeneration.
Keyword Biotechnology & Applied Microbiology
Plant Sciences
Agrobacterium Tumefaciens
Etr1-1
Intron Gus
Lactuca Sativa L.
Lba4404
Transformation
Arabidopsis
Senescence
Cuttings
Ethephon
Plants
Q-Index Code C1
Institutional Status UQ

 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 7 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 9 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Wed, 15 Aug 2007, 13:28:07 EST