Nucleic acid purification from plants, animals and microbes in under 30 seconds

Zou, Yiping, Mason, Michael Glenn, Wang, Yuling, Wee, Eugene, Turni, Conny, Blackall, Patrick J., Trau, Matt and Botella, Jose Ramon (2017) Nucleic acid purification from plants, animals and microbes in under 30 seconds. PLoS Biology, 15 11: e2003916. doi:10.1371/journal.pbio.2003916


Author Zou, Yiping
Mason, Michael Glenn
Wang, Yuling
Wee, Eugene
Turni, Conny
Blackall, Patrick J.
Trau, Matt
Botella, Jose Ramon
Title Nucleic acid purification from plants, animals and microbes in under 30 seconds
Journal name PLoS Biology   Check publisher's open access policy
ISSN 1544-9173
1545-7885
Publication date 2017-11-21
Year available 2017
Sub-type Article (original research)
DOI 10.1371/journal.pbio.2003916
Open Access Status DOI
Volume 15
Issue 11
Start page e2003916
Total pages 22
Place of publication San Francisco, CA, United States
Publisher Public Library of Science
Language eng
Subject 2800 Neuroscience
1300 Biochemistry, Genetics and Molecular Biology
2400 Immunology and Microbiology
1100 Agricultural and Biological Sciences
Abstract Nucleic acid amplification is a powerful molecular biology tool, although its use outside the modern laboratory environment is limited due to the relatively cumbersome methods required to extract nucleic acids from biological samples. To address this issue, we investigated a variety of materials for their suitability for nucleic acid capture and purification. We report here that untreated cellulose-based paper can rapidly capture nucleic acids within seconds and retain them during a single washing step, while contaminants present in complex biological samples are quickly removed. Building on this knowledge, we have successfully created an equipment-free nucleic acid extraction dipstick methodology that can obtain amplification-ready DNA and RNA from plants, animals, and microbes from difficult biological samples such as blood and leaves from adult trees in less than 30 seconds. The simplicity and speed of this method as well as the low cost and availability of suitable materials (e.g., common paper towelling), means that nucleic acid extraction is now more accessible and affordable for researchers and the broader community. Furthermore, when combined with recent advancements in isothermal amplification and naked eye DNA visualization techniques, the dipstick extraction technology makes performing molecular diagnostic assays achievable in limited resource settings including university and high school classrooms, field-based environments, and developing countries.
Keyword Real-Time Pcr
Dna Extraction Method
Whole-Blood
Molecular Diagnosis
Cellulose Chromatography
Colorimetric Detection
Sample Preparation
Escherichia-Coli
Proviral Dna
Low-Cost
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

 
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Created: Fri, 01 Dec 2017, 15:37:44 EST by Dr Michael Mason on behalf of School of Agriculture and Food Sciences