Transcriptome profile of lung dendritic cells after in vitro porcine reproductive and respiratory syndrome virus (PRRSV) infection

Pröll, Maren Julia, Neuhoff, Christiane, Schellander, Karl, Uddin, Muhammad Jasim, Cinar, Mehmet Ulas, Sahadevan, Sudeep, Qu, Xueqi, Islam, Md Aminul, Poirier, Mikhael, Müller, Marcel A, Drosten, Christian, Tesfaye, Dawit, Tholen, Ernst and Große-Brinkhaus, Christine (2017) Transcriptome profile of lung dendritic cells after in vitro porcine reproductive and respiratory syndrome virus (PRRSV) infection. PLoS One, 12 11: e0187735. doi:10.1371/journal.pone.0187735


Author Pröll, Maren Julia
Neuhoff, Christiane
Schellander, Karl
Uddin, Muhammad Jasim
Cinar, Mehmet Ulas
Sahadevan, Sudeep
Qu, Xueqi
Islam, Md Aminul
Poirier, Mikhael
Müller, Marcel A
Drosten, Christian
Tesfaye, Dawit
Tholen, Ernst
Große-Brinkhaus, Christine
Title Transcriptome profile of lung dendritic cells after in vitro porcine reproductive and respiratory syndrome virus (PRRSV) infection
Journal name PLoS One   Check publisher's open access policy
ISSN 1932-6203
Publication date 2017-11-15
Year available 2017
Sub-type Article (original research)
DOI 10.1371/journal.pone.0187735
Open Access Status DOI
Volume 12
Issue 11
Start page e0187735
Total pages 20
Place of publication San Francisco, CA., United States
Publisher Public Library of Science
Language eng
Subject 1300 Biochemistry, Genetics and Molecular Biology
1100 Agricultural and Biological Sciences
Abstract The porcine reproductive and respiratory syndrome (PRRS) is an infectious disease that leads to high financial and production losses in the global swine industry. The pathogenesis of this disease is dependent on a multitude of factors, and its control remains problematic. The immune system generally defends against infectious diseases, especially dendritic cells (DCs), which play a crucial role in the activation of the immune response after viral infections. However, the understanding of the immune response and the genetic impact on the immune response to PRRS virus (PRRSV) remains incomplete. In light of this, we investigated the regulation of the host immune response to PRRSV in porcine lung DCs using RNA-sequencing (RNA-Seq). Lung DCs from two different pig breeds (Pietrain and Duroc) were collected before (0 hours) and during various periods of infection (3, 6, 9, 12, and 24 hours post infection (hpi)). RNA-Seq analysis revealed a total of 20,396 predicted porcine genes, which included breed-specific differentially expressed immune genes. Pietrain and Duroc infected lung DCs showed opposite gene expression courses during the first time points post infection. Duroc lung DCs reacted more strongly and distinctly than Pietrain lung DCs during these periods (3, 6, 9, 12 hpi). Additionally, cluster analysis revealed time-dependent co-expressed groups of genes that were involved in immune-relevant pathways. Key clusters and pathways were identified, which help to explain the biological and functional background of lung DCs post PRRSV infection and suggest IL-1β1 as an important candidate gene. RNA-Seq was also used to characterize the viral replication of PRRSV for each breed. PRRSV was able to infect and to replicate differently in lung DCs between the two mentioned breeds. These results could be useful in investigations on immunity traits in pig breeding and enhancing the health of pigs.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
School of Veterinary Science Publications
 
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Created: Wed, 29 Nov 2017, 12:03:54 EST