Gene expression during early ascidian metamorphosis requires signaling by Hemps, an EGF-like protein

Woods, R. G., Roper, K. E., Gauthier, M., Bebell, L. M., Sung, K., Degnan, B. M. and Lavin, M. F. (2004) Gene expression during early ascidian metamorphosis requires signaling by Hemps, an EGF-like protein. Development, 131 12: 2921-2933. doi:10.1242/dev.01120

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Author Woods, R. G.
Roper, K. E.
Gauthier, M.
Bebell, L. M.
Sung, K.
Degnan, B. M.
Lavin, M. F.
Title Gene expression during early ascidian metamorphosis requires signaling by Hemps, an EGF-like protein
Journal name Development   Check publisher's open access policy
ISSN 0950-1991
Publication date 2004-01-01
Sub-type Article (original research)
DOI 10.1242/dev.01120
Open Access Status File (Publisher version)
Volume 131
Issue 12
Start page 2921
End page 2933
Total pages 13
Editor J. Smith
Place of publication Cambridge, U.K.
Publisher Company of Biologists
Language eng
Subject C1
321204 Mental Health
730204 Child health
270200 Genetics
270201 Gene Expression
270500 Zoology
Abstract Hemps, a novel epidermal growth factor (EGF)-like protein, is expressed during larval development and early metamorphosis in the ascidian Herdmania curvata and plays a direct role in triggering metamorphosis. In order to identify downstream genes in the Hemps pathway we used a gene expression profiling approach, in which we compared post-larvae undergoing normal metamorphosis with larval metamorphosis blocked with an anti-Hemps antibody. Molecular profiling revealed that there are dynamic changes in gene expression within the first 30 minutes of normal metamorphosis with a significant portion of the genome (approximately 49%) being activated or repressed. A more detailed analysis of the expression of 15 of these differentially expressed genes through embryogenesis, larval development and metamorphosis revealed that while there is a diversity of temporal expression patterns, a number of genes are transiently expressed during larval development and metamorphosis. These and other differentially expressed genes were localised to a range of specific cell and tissue types in Herdmania larvae and post-larvae. The expression of approximately 24% of the genes that were differentially expressed during early metamorphosis was affected in larvae treated with the anti-Hemps antibody. Knockdown of Hemps activity affected the expression of a range of genes within 30 minutes of induction, suggesting that the Hemps pathway directly regulates early response genes at metamorphosis. In most cases, it appears that the Hemps pathway contributes to the modulation of gene expression, rather than initial gene activation or repression. A total of 151 genes that displayed the greatest alterations in expression in response to anti-Hemps antibody were sequenced. These genes were implicated in a range of developmental and physiological roles, including innate immunity, signal transduction and in the regulation of gene transcription. These results suggest that there is significant gene activity during the very early stages of H. curvata metamorphosis and that the Hemps pathway plays a key role in regulating the expression of many of these genes.
Keyword Developmental Biology
Hemps
Ascidian Herdmania Curvata
Gene Expression Profiling
Ciona-intestinalis Metamorphosis
Factor-like Repeats
Developmental Fates
Halocynthia-roretzi
Herdmania-momus
Larval Tissues
Retinoic Acid
Body Plans
Growth
Induction
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
2005 Higher Education Research Data Collection
School of Medicine Publications
 
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Created: Wed, 15 Aug 2007, 13:24:02 EST