RNA binding protein Musashi-2 regulates PIWIL1 and TBX1 in mouse spermatogenesis

Sutherland, Jessie M., Sobinoff, Alexander P., Fraser, Barbara A., Redgrove, Kate A., Siddall, Nicole A., Koopman, Peter, Hime, Gary R. and McLaughlin, Eileen A. (2018) RNA binding protein Musashi-2 regulates PIWIL1 and TBX1 in mouse spermatogenesis. Journal of Cellular Physiology, 233 4: 3262-3273. doi:10.1002/jcp.26168

Author Sutherland, Jessie M.
Sobinoff, Alexander P.
Fraser, Barbara A.
Redgrove, Kate A.
Siddall, Nicole A.
Koopman, Peter
Hime, Gary R.
McLaughlin, Eileen A.
Title RNA binding protein Musashi-2 regulates PIWIL1 and TBX1 in mouse spermatogenesis
Journal name Journal of Cellular Physiology   Check publisher's open access policy
ISSN 1097-4652
Publication date 2018-04-01
Sub-type Article (original research)
DOI 10.1002/jcp.26168
Open Access Status Not yet assessed
Volume 233
Issue 4
Start page 3262
End page 3273
Total pages 12
Place of publication Hoboken, NJ, United States
Publisher John Wiley & Sons
Language eng
Subject 1314 Physiology
1308 Clinical Biochemistry
1307 Cell Biology
Abstract RNA-binding proteins (RBP) are important facilitators of post-transcriptional gene regulation. We have previously established that nuclear overexpression of the RBP Musashi-2 (MSI2) during male germ cell maturation is detrimental to sperm cell development and fertility. Herein we determine the genes and pathways impacted by the upregulation of Msi2. Microarray analysis and qPCR confirmed differential gene expression in factors fundamental to the cell cycle, cellular proliferation, and cell death. Similarly, comparative protein expression analysis via iTRAQ, immunoblot, and immunolocalization, identified differential expression and localization of important regulators of transcription, translation, RNA processing, and spermatogenesis. Specifically, the testis-expressed transcription factor, Tbx1, and the piRNA regulator of gamete development, Piwil1, were both found to be targeted for translational repression by MSI2. This study provides key evidence to support a fundamental role for MSI2 in post-transcriptional regulation during male gamete development.
Keyword MSI2
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
Institute for Molecular Bioscience - Publications
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Created: Wed, 15 Nov 2017, 12:24:30 EST