Unlocking new alleles for leaf rust resistance in the Vavilov wheat collection

Riaz, Adnan, Athiyannan, Naveenkumar, Periyannan, Sambasivam K, Afanasenko, Olga, Mitrofanova, Olga P, Platz, Gregory J, Aitken, Elizabeth A B, Snowdon, Rod J, Lagudah, Evans S, Hickey, Lee T and Voss-Fels, Kai P (2017) Unlocking new alleles for leaf rust resistance in the Vavilov wheat collection. TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 131 1: 127-144. doi:10.1007/s00122-017-2990-5


Author Riaz, Adnan
Athiyannan, Naveenkumar
Periyannan, Sambasivam K
Afanasenko, Olga
Mitrofanova, Olga P
Platz, Gregory J
Aitken, Elizabeth A B
Snowdon, Rod J
Lagudah, Evans S
Hickey, Lee T
Voss-Fels, Kai P
Title Unlocking new alleles for leaf rust resistance in the Vavilov wheat collection
Journal name TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik   Check publisher's open access policy
ISSN 1432-2242
Publication date 2017-10-04
Sub-type Article (original research)
DOI 10.1007/s00122-017-2990-5
Open Access Status Not yet assessed
Volume 131
Issue 1
Start page 127
End page 144
Total pages 18
Abstract Thirteen potentially new leaf rust resistance loci were identified in a Vavilov wheat diversity panel. We demonstrated the potential of allele stacking to strengthen resistance against this important pathogen. Leaf rust (LR) caused by Puccinia triticina is an important disease of wheat (Triticum aestivum L.), and the deployment of genetically resistant cultivars is the most viable strategy to minimise yield losses. In this study, we evaluated a diversity panel of 295 bread wheat accessions from the N. I. Vavilov Institute of Plant Genetic Resources (St Petersburg, Russia) for LR resistance and performed genome-wide association studies (GWAS) using 10,748 polymorphic DArT-seq markers. The diversity panel was evaluated at seedling and adult plant growth stages using three P. triticina pathotypes prevalent in Australia. GWAS was applied to 11 phenotypic data sets which identified a total of 52 significant marker-trait associations representing 31 quantitative trait loci (QTL). Among them, 29 QTL were associated with adult plant resistance (APR). Of the 31 QTL, 13 were considered potentially new loci, whereas 4 co-located with previously catalogued Lr genes and 14 aligned to regions reported in other GWAS and genomic prediction studies. One seedling LR resistance QTL located on chromosome 3A showed pronounced levels of linkage disequilibrium among markers (r (2) = 0.7), suggested a high allelic fixation. Subsequent haplotype analysis for this region found seven haplotype variants, of which two were strongly associated with LR resistance at seedling stage. Similarly, analysis of an APR QTL on chromosome 7B revealed 22 variants, of which 4 were associated with resistance at the adult plant stage. Furthermore, most of the tested lines in the diversity panel carried 10 or more combined resistance-associated marker alleles, highlighting the potential of allele stacking for long-lasting resistance.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Pubmed Import
 
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Created: Wed, 15 Nov 2017, 12:17:24 EST