The effect of bisphosphonates on the endothelial differentiation of mesenchymal stem cells

Sharma, Dileep, Hamlet, Stephen Mark, Petcu, Eugen Bogdan and Ivanovski, Saso (2016) The effect of bisphosphonates on the endothelial differentiation of mesenchymal stem cells. Scientific Reports, 6 . doi:10.1038/srep20580

Author Sharma, Dileep
Hamlet, Stephen Mark
Petcu, Eugen Bogdan
Ivanovski, Saso
Title The effect of bisphosphonates on the endothelial differentiation of mesenchymal stem cells
Journal name Scientific Reports   Check publisher's open access policy
ISSN 2045-2322
Publication date 2016-02-01
Year available 2016
Sub-type Article (original research)
DOI 10.1038/srep20580
Open Access Status DOI
Volume 6
Total pages 11
Place of publication London, United Kingdom
Publisher Nature Publishing Group
Language eng
Subject 1000 General
Abstract The contribution of the local stem cell niche to providing an adequate vascular framework during healing cannot be overemphasized. Bisphosphonates (BPs) are known to have a direct effect on the local vasculature, but their effect on progenitor cell differentiation is unknown. This in vitro study evaluated the effect(s) of various BPs on the differentiation of human placental mesenchymal stem cells (pMSCs) along the endothelial lineage and their subsequent functional and morphogenic capabilities. pMSC multipotency was confirmed by successful differentiation into cells of both the osteogenic and endothelial lineages, as demonstrated by positive Alizarin Red S staining and Ac-LDL uptake. pMSC differentiation in the presence of non-cytotoxic BP concentrations showed that nitrogen containing BPs had a significant inhibitory effect on cell migration and endothelial marker gene expression, as well as compromised endothelial differentiation as demonstrated using von Willebrand factor immunofluorescence staining and tube formation assay. This in vitro study demonstrated that at non-cytotoxic levels, nitrogen-containing BPs inhibit differentiation of pMSCs into cells of an endothelial lineage and affect the downstream functional capability of these cells supporting a multi-modal effect of BPs on angiogenesis as pathogenic mechanism contributing to bone healing disorders such as bisphosphonate related osteonecrosis of the jaws (BRONJ).
Keyword Stromal Cells
Q-Index Code C1
Q-Index Status Provisional Code
Grant ID 86-2014
Institutional Status Non-UQ
Additional Notes Article number 20580

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
School of Dentistry Publications
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