The influence of cellular source on periodontal regeneration using calcium phosphate coated polycaprolactone scaffold supported cell sheets

Dan, Hongxia, Vaquette, Cedryck, Fisher, Anthony G., Hamlet, Stephen M., Xiao, Yin, Hutmacher, Dietmar W. and Ivanovski, Saso (2014) The influence of cellular source on periodontal regeneration using calcium phosphate coated polycaprolactone scaffold supported cell sheets. Biomaterials, 35 1: 113-122. doi:10.1016/j.biomaterials.2013.09.074


Author Dan, Hongxia
Vaquette, Cedryck
Fisher, Anthony G.
Hamlet, Stephen M.
Xiao, Yin
Hutmacher, Dietmar W.
Ivanovski, Saso
Title The influence of cellular source on periodontal regeneration using calcium phosphate coated polycaprolactone scaffold supported cell sheets
Journal name Biomaterials   Check publisher's open access policy
ISSN 1878-5905
0142-9612
Publication date 2014-01-01
Year available 2014
Sub-type Article (original research)
DOI 10.1016/j.biomaterials.2013.09.074
Open Access Status Not yet assessed
Volume 35
Issue 1
Start page 113
End page 122
Total pages 10
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Language eng
Abstract Cell-based therapy is considered a promising approach to achieving predictable periodontal regeneration. In this study, the regenerative potential of cell sheets derived from different parts of the periodontium (gingival connective tissue, alveolar bone and periodontal ligament) were investigated in an athymic rat periodontal defect model. Periodontal ligament (PDLC), alveolar bone (ABC) and gingival margin-derived cells (GMC) were obtained from human donors. The osteogenic potential of the primary cultures was demonstrated in vitro. Cell sheets supported by a calcium phosphate coated melt electrospun polycaprolactone (CaP-PCL) scaffold were transplanted to denuded root surfaces in surgically created periodontal defects, and allowed to heal for 1 and 4 weeks. The CaP-PCL scaffold alone was able to promote alveolar bone formation within the defect after 4 weeks. The addition of ABC and PDLC sheets resulted in significant periodontal attachment formation. The GMC sheets did not promote periodontal regeneration on the root surface and inhibited bone formation within the CaP-PCL scaffold. In conclusion, the combination of either PDLC or ABC sheets with a CaP-PCL scaffold could promote periodontal regeneration, but ABC sheets were not as effective as PDLC sheets in promoting new attachment formation. (C) 2013 Elsevier Ltd. All rights reserved.
Keyword Alveolar bone-derived cell
Cell sheet
Gingival margin-derived cell
PCL scaffold
Periodontal ligament cell
Periodontal regeneration
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Dentistry Publications
 
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