The microRNA expression signature on modified titanium implant surfaces influences genetic mechanisms leading to osteogenic differentiation

Chakravorty, Nishant, Ivanovski, Saso, Prasadam, Indira, Crawford, Ross, Oloyede, Adekunle and Xiao, Yin (2012) The microRNA expression signature on modified titanium implant surfaces influences genetic mechanisms leading to osteogenic differentiation. Acta Biomaterialia, 8 9: 3516-3523. doi:10.1016/j.actbio.2012.05.008


Author Chakravorty, Nishant
Ivanovski, Saso
Prasadam, Indira
Crawford, Ross
Oloyede, Adekunle
Xiao, Yin
Title The microRNA expression signature on modified titanium implant surfaces influences genetic mechanisms leading to osteogenic differentiation
Journal name Acta Biomaterialia   Check publisher's open access policy
ISSN 1878-7568
1742-7061
Publication date 2012-09-01
Year available 2012
Sub-type Article (original research)
DOI 10.1016/j.actbio.2012.05.008
Open Access Status Not yet assessed
Volume 8
Issue 9
Start page 3516
End page 3523
Total pages 8
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Language eng
Abstract Topographically and chemically modified titanium implants are recognized to have improved osteogenic properties; however, the molecular regulation of this process remains unknown. This study aimed to determine the microRNA profile and the potential regulation of osteogenic differentiation following early exposure of osteoprogenitor cells to sand-blasted, large-grit acid-etched (SLA) and hydrophilic SLA (mod-SLA) surfaces. Firstly, the osteogenic characteristics of the primary osteoprogenitor cells were confirmed using ALP activity and Alizarin Red S staining. The effect of smooth (SMO). SLA and modSLA surfaces on the TGF-beta/BMP (BMP2, BMP6, ACVR1) and non-canonical WNT/Ca2+ (WNT5A, FZD6) pathways, as well as the integrins ITGB1 and ITGA2, was determined. It was revealed that the modified titanium surfaces could induce the activation of TGF-beta/BMP and non-canonical WNT/Ca2+ signaling genes. The expression pattern of microRNAs (miRNAs) related to cell differentiation was evaluated. Statistical analysis of the differentially regulated miRNAs indicated that 35 and 32 miRNAs were down-regulated on the modSLA and SLA surfaces respectively, when compared with the smooth surface (SMO). Thirty-one miRNAs that were down-regulated were common to both modSLA and SLA. There were 10 miRNAs up-regulated on modSLA and nine on SLA surfaces, amongst which eight were the same as observed on modSLA. TargetScan predictions for the down-regulated miRNAs revealed genes of the TGF-beta/BMP and non-canonical Ca2+ pathways as targets. This study demonstrated that modified titanium implant surfaces induce differential regulation of miRNAs, which potentially regulate the TGF-beta/BMP and WNT/Ca2+ pathways during osteogenic differentiation on modified titanium implant surfaces. (C) 2012 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
Keyword Gene expression
MicroRNA
Osteoblast differentiation
Surface modification
Titanium
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Dentistry Publications
 
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