Serum bone formation marker correlation with improved osseointegration in osteoporotic rats treated with simvastatin

Du, Zhibin, Chen, Jiang, Yan, Fuhua, Doan, Nghiem, Ivanovski, Saso and Xiao, Yin (2013) Serum bone formation marker correlation with improved osseointegration in osteoporotic rats treated with simvastatin. Clinical Oral Implants Research, 24 4: 422-427. doi:10.1111/j.1600-0501.2011.02341.x


Author Du, Zhibin
Chen, Jiang
Yan, Fuhua
Doan, Nghiem
Ivanovski, Saso
Xiao, Yin
Title Serum bone formation marker correlation with improved osseointegration in osteoporotic rats treated with simvastatin
Journal name Clinical Oral Implants Research   Check publisher's open access policy
ISSN 1600-0501
0905-7161
Publication date 2013-04-01
Year available 2013
Sub-type Article (original research)
DOI 10.1111/j.1600-0501.2011.02341.x
Open Access Status Not yet assessed
Volume 24
Issue 4
Start page 422
End page 427
Total pages 6
Place of publication Hoboken, NJ, United States
Publisher Wiley-Blackwell Publishing
Language eng
Abstract Objective Simvastatin has been shown to enhance osseointegration of pure titanium implants in osteoporotic rats. This study aimed to evaluate the relationship between the serum level of bone formation markers and the osseointegration of pure titanium implants in osteoporotic rats treated with simvastatin. Materials and methods Fifty-four female Sprague-Dawley rats, aged 3months, were randomly divided into three groups: Sham-operated group (SHAM; n=18), ovariectomized group (OVX; n=18), and ovariectomized with simvastatin treatment group (OVX+SIM; n=18). Fifty-six days after ovariectomy, screw-shaped titanium implants were inserted into the tibiae. Simvastatin was administered orally at 5mg/kg each day after the placement of the implant in the OVX+SIM group. The animals were killed at either 28 or 84days after implantation and the undecalcified tissue sections were processed for histological analysis. Total alkaline phosphatase (ALP), bone-specific alkaline phosphatase (BALP) and bone Gla protein (BGP) were measured in all animal sera collected at the time of euthanasia and correlated with the histological assessment of osseointegration. Results The level of ALP in the OVX group was higher than the SHAM group at day 28, with no differences between the three groups at day 84. The level of BALP in the OVX+SIM group was significantly higher than both OVX and SHAM groups at days 28. Compared with day 28, the BALP level of all three groups showed a significant decrease at day 84. There were no significant differences in BGP levels between the three groups at day 28, but at day 84, the OVX+ SIM group showed significantly higher levels than both the OVX and SHAM groups. There was a significant increase in BGP levels between days 28 and 84 in the OVX+SIM group. The serum bone marker levels correlated with the histological assessment showing reduced osseointegration in the OVX compared to the SHAM group which is subsequently reversed in the OVX+SIM group. Conclusion The results from this study indicate that the serum level of bone formation markers, especially BALP and BGP, could be correlated with the degree of osseointegration around titanium implants in osteoporotic rats treated with simvastatin.
Keyword Osseointegration
Osteoporosis
Serum bone formation markers
Simvastatin
Titanium implants
Q-Index Code C1
Q-Index Status Provisional Code
Grant ID 2010H6009
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Dentistry Publications
 
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