The vegetative nitrogen response of sorghum lines containing different alleles for nitrate reductase and glutamate synthase

Diatloff, Eugene, Mace, Emma S., Jordan, David R., Filleur, Sophie, Tai, Shuaishuai, Schmidt, Susanne and Godwin, Ian D. (2017) The vegetative nitrogen response of sorghum lines containing different alleles for nitrate reductase and glutamate synthase. Molecular Breeding, 37 11: . doi:10.1007/s11032-017-0738-1


Author Diatloff, Eugene
Mace, Emma S.
Jordan, David R.
Filleur, Sophie
Tai, Shuaishuai
Schmidt, Susanne
Godwin, Ian D.
Title The vegetative nitrogen response of sorghum lines containing different alleles for nitrate reductase and glutamate synthase
Journal name Molecular Breeding   Check publisher's open access policy
ISSN 1380-3743
1572-9788
Publication date 2017-10-25
Year available 2017
Sub-type Article (original research)
DOI 10.1007/s11032-017-0738-1
Open Access Status Not yet assessed
Volume 37
Issue 11
Total pages 14
Place of publication Dordrecht,The Netherlands
Publisher Springer
Language eng
Abstract Improving the nitrogen (N) responsiveness of crops is crucial for food security and environmental sustainability, and breeding N use efficient (NUE) crops has to exploit genetic variation for this complex trait. We used reverse genetics to examine allelic variation in two N metabolism genes. In silico analysis of the genomes of 44 genetically diverse sorghum genotypes identified a nitrate reductase and a glutamate synthase gene that were under balancing selection in improved sorghum cultivars. We hypothesised that these genes are a potential source of differences in NUE, and selected parents and progeny of nested association mapping populations with different allelic combinations for these genes. Allelic variation was sourced from African (Macia) and Indian (ICSV754) genotypes that had been incorporated into the Australian elite parent R931945-2-2. Nine genotypes were grown for 30 days in a glasshouse and supplied with continuous limiting or replete N, or replete N for 27 days followed by 3 days of N starvation. Biomass, total N and nitrate contents were quantified together with gene expressions in leaves, stems and roots. Limiting N supply universally resulted in less shoot and root growth, increased root weight ratio and reduced tissue nitrate and total N concentrations. None of the tested genotypes exceeded growth or NUE of the elite parent R931945-2-2 indicating that the allelic combinations did not confer an advantage during early vegetative growth. Thus, the next steps for ascertaining potential effects on NUE include growing plants to maturity. We conclude that reverse genetics that take advantage of rapidly expanding genomic databases enable a systematic approach for developing N-efficient crops.
Keyword Use Efficiency
Genetic-Variability
Balancing Selection
Leaf Expansion
Plants
Crop
Nutrition
Evolution
Genotypes
Bicolor
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
School of Agriculture and Food Sciences
Queensland Alliance for Agriculture and Food Innovation
 
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Created: Sat, 11 Nov 2017, 23:05:32 EST