An immortalized adult human erythroid line facilitates sustainable and scalable generation of functional red cells

Trakarnsanga, Kongtana, Griffiths, Rebecca E., Wilson, Marieangela C., Blair, Allison, Satchwell, Timothy J., Meinders, Marjolein, Cogan, Nicola, Kupzig, Sabine, Kurita, Ryo, Nakamura, Yukio, Toye, Ashley M., Anstee, David J. and Frayne, Jan (2017) An immortalized adult human erythroid line facilitates sustainable and scalable generation of functional red cells. Nature Communications, 8 14750. doi:10.1038/ncomms14750


Author Trakarnsanga, Kongtana
Griffiths, Rebecca E.
Wilson, Marieangela C.
Blair, Allison
Satchwell, Timothy J.
Meinders, Marjolein
Cogan, Nicola
Kupzig, Sabine
Kurita, Ryo
Nakamura, Yukio
Toye, Ashley M.
Anstee, David J.
Frayne, Jan
Title An immortalized adult human erythroid line facilitates sustainable and scalable generation of functional red cells
Journal name Nature Communications   Check publisher's open access policy
ISSN 2041-1723
Publication date 2017-03-14
Sub-type Article (original research)
DOI 10.1038/ncomms14750
Open Access Status DOI
Volume 8
Start page 14750
Publisher Nature Publishing Group
Language eng
Subject 1600 Chemistry
1300 Biochemistry, Genetics and Molecular Biology
3100 Physics and Astronomy
Abstract With increasing worldwide demand for safe blood, there is much interest in generating red blood cells in vitro as an alternative clinical product. However, available methods for in vitro generation of red cells from adult and cord blood progenitors do not yet provide a sustainable supply, and current systems using pluripotent stem cells as progenitors do not generate viable red cells. We have taken an alternative approach, immortalizing early adult erythroblasts generating a stable line, which provides a continuous supply of red cells. The immortalized cells differentiate efficiently into mature, functional reticulocytes that can be isolated by filtration. Extensive characterization has not revealed any differences between these reticulocytes and in vitro-cultured adult reticulocytes functionally or at the molecular level, and importantly no aberrant protein expression. We demonstrate a feasible approach to the manufacture of red cells for clinical use from in vitro culture.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import
 
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Created: Fri, 03 Nov 2017, 16:53:29 EST