A mouse model of hyperproliferative human epithelium validated by keratin profiling shows an aberrant cytoskeletal response to injury

Zhussupbekova, Samal, Sinha, Rohit, Kuo, Paula, Lambert, Paul F., Frazer, Ian H. and Tuong, Zewen K. (2016) A mouse model of hyperproliferative human epithelium validated by keratin profiling shows an aberrant cytoskeletal response to injury. EBioMedicine, 9 314-323. doi:10.1016/j.ebiom.2016.06.011


Author Zhussupbekova, Samal
Sinha, Rohit
Kuo, Paula
Lambert, Paul F.
Frazer, Ian H.
Tuong, Zewen K.
Title A mouse model of hyperproliferative human epithelium validated by keratin profiling shows an aberrant cytoskeletal response to injury
Journal name EBioMedicine   Check publisher's open access policy
ISSN 2352-3964
Publication date 2016-07-01
Year available 2016
Sub-type Article (original research)
DOI 10.1016/j.ebiom.2016.06.011
Open Access Status DOI
Volume 9
Start page 314
End page 323
Total pages 10
Place of publication Amsterdam, Netherlands
Publisher Elsevier BV
Language eng
Abstract A validated animal model would assist with research on the immunological consequences of the chronic expression of stress keratins KRT6, KRT16, and KRT17, as observed in human pre-malignant hyperproliferative epithelium. Here we examine keratin gene expression profile in skin from mice expressing the E7 oncoprotein of HPV16 (K14E7) demonstrating persistently hyperproliferative epithelium, in nontransgenic mouse skin, and in hyperproliferative actinic keratosis lesions from human skin. We demonstrate that K14E7 mouse skin overexpresses stress keratins in a similar manner to human actinic keratoses, that overexpression is a consequence of epithelial hyperproliferation induced by E7, and that overexpression further increases in response to injury. As stress keratins modify local immunity and epithelial cell function and differentiation, the K14E7 mouse model should permit study of how continued overexpression of stress keratins impacts on epithelial tumor development and on local innate and adaptive immunity.
Keyword E7
HPV16
Hyperplasia
Keratins
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
UQ Diamantina Institute Publications
 
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Created: Fri, 03 Nov 2017, 15:51:14 EST