Gold-loaded nanoporous ferric oxide nanocubes with peroxidase-mimicking activity for electrocatalytic and colorimetric Detection of autoantibody

Masud, Mostafa Kamal, Yadav, Sharda, Islam, Md. Nazmul, Nguyen, Nam-Trung, Salomon, Carlos, Kline, Richard, Alamri, Hatem R., Alothman, Zeid A., Yamauchi, Yusuke, Hossain, Md. Shahriar A. and Shiddiky, Muhammad J. A. (2017) Gold-loaded nanoporous ferric oxide nanocubes with peroxidase-mimicking activity for electrocatalytic and colorimetric Detection of autoantibody. Analytical Chemistry, 89 20: 11005-11013. doi:10.1021/acs.analchem.7b02880


Author Masud, Mostafa Kamal
Yadav, Sharda
Islam, Md. Nazmul
Nguyen, Nam-Trung
Salomon, Carlos
Kline, Richard
Alamri, Hatem R.
Alothman, Zeid A.
Yamauchi, Yusuke
Hossain, Md. Shahriar A.
Shiddiky, Muhammad J. A.
Title Gold-loaded nanoporous ferric oxide nanocubes with peroxidase-mimicking activity for electrocatalytic and colorimetric Detection of autoantibody
Journal name Analytical Chemistry   Check publisher's open access policy
ISSN 1520-6882
0003-2700
Publication date 2017-10-17
Year available 2017
Sub-type Article (original research)
DOI 10.1021/acs.analchem.7b02880
Open Access Status Not yet assessed
Volume 89
Issue 20
Start page 11005
End page 11013
Total pages 9
Place of publication Washington, DC., United States
Publisher American Chemical Society
Language eng
Subject 1602 Analytical Chemistry
Abstract (Graph Presented) The enzyme-mimicking activity of iron oxide based nanostructures has provided a significant advantage in developing advanced molecular sensors for biomedical and environmental applications. Herein, we introduce the horseradish peroxidase (HRP)-like activity of gold-loaded nanoporous ferric oxide nanocubes (Au-NPFeONC) for the development of a molecular sensor with enhanced electrocatalytic and colorimetric (naked eye) detection of autoantibodies. The results showed that Au-NPFeONC exhibits enhanced peroxidase-like activity toward the catalytic oxidation of 3,3′,5,5′-tertamethylbenzidine (TMB) in the presence of HO at room temperature (25 °C) and follows the typical Michaelis-Menten kinetics. The autoantibody sensor based on this intrinsic property of Au-NPFeONC resulted in excellent detection sensitivity [limit of detection (LOD) = 0.08 U/mL] and reproducibility [percent relative standard deviation (% RSD) = <5% for n = 3] for analyzing p53-specific autoantibodies using electrochemical and colorimetric (naked eye) readouts. The clinical applicability of the sensor has been tested in detecting p53-specific autoantibody in plasma obtained from patients with epithelial ovarian cancer high-grade serous subtype (EOCHGS, number of samples = 2) and controls (benign, number of samples = 2). As Au-NPFeONC possess high peroxidase-like activity for the oxidation of TMB in the presence of HO [TMB is a common chromogenic substrate for HRP in enzyme-linked immunosorbent assays (ELISAs)], we envisage that our assay could find a wide range of application in developing ELISA-based sensing approaches in the fields of medicine (i.e., detection of other biomarkers the same as p53 autoantibody), biotechnology, and environmental sciences.
Keyword Serous Ovarian-Cancer
Magnetic Nanoparticles
Affinity Interactions
Catalyzed Oxidation
P53 Autoantibodies
Colorectal-Cancer
Early-Diagnosis
Glucose
Biomarkers
Antibodies
Q-Index Code C1
Q-Index Status Provisional Code
Grant ID APP1088966
FONDECYT 1170809
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: School of Chemical Engineering Publications
HERDC Pre-Audit
Australian Institute for Bioengineering and Nanotechnology Publications
 
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Created: Fri, 03 Nov 2017, 09:10:07 EST