Progressive sheet-to-tubule transformation is a general mechanism for endoplasmic reticulum partitioning in dividing mammalian cells

Puhka, Maija, Joensuu, Merja, Vihinen, Helena, Belevich, Ilya and Jokitalo, Eija (2012) Progressive sheet-to-tubule transformation is a general mechanism for endoplasmic reticulum partitioning in dividing mammalian cells. Molecular Biology of the Cell, 23 13: 2424-2432. doi:10.1091/mbc.E10-12-0950

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Author Puhka, Maija
Joensuu, Merja
Vihinen, Helena
Belevich, Ilya
Jokitalo, Eija
Title Progressive sheet-to-tubule transformation is a general mechanism for endoplasmic reticulum partitioning in dividing mammalian cells
Journal name Molecular Biology of the Cell   Check publisher's open access policy
ISSN 1059-1524
1939-4586
Publication date 2012-07-01
Sub-type Article (original research)
DOI 10.1091/mbc.E10-12-0950
Open Access Status File (Publisher version)
Volume 23
Issue 13
Start page 2424
End page 2432
Total pages 9
Place of publication Bethesda, MD United States
Publisher American Society for Cell Biology
Language eng
Abstract The endoplasmic reticulum (ER) is both structurally and functionally complex, consisting of a dynamic network of interconnected sheets and tubules. To achieve a more comprehensive view of ER organization in interphase and mitotic cells and to address a discrepancy in the field (i.e., whether ER sheets persist, or are transformed to tubules, during mitosis), we analyzed the ER in four different mammalian cell lines using live-cell imaging, high-resolution electron microscopy, and three dimensional electron microscopy. In interphase cells, we found great variation in network organization and sheet structures among different cell lines. In mitotic cells, we show that the ER undergoes both spatial reorganization and structural transformation of sheets toward more fenestrated and tubular forms. However, the extent of spatial reorganization and sheet-to-tubule transformation varies among cell lines. Fenestration and tubulation of the ER correlates with a reduced number of membrane-bound ribosomes.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Queensland Brain Institute Publications
 
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