ER sheet persistence is coupled to myosin 1c-regulated dynamic actin filament arrays

Joensuu, Merja, Belevich, Ilya, Ramo, Olli, Nevzorov, Ilya, Vihinen, Helena, Puhka, Maija, Witkos, Tomasz M., Lowe, Martin, Vartiainen, Maria K. and Jokitalo, Eija (2014) ER sheet persistence is coupled to myosin 1c-regulated dynamic actin filament arrays. Molecular Biology of the Cell, 25 7: 1111-1126. doi:10.1091/mbc.E13-12-0712

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Author Joensuu, Merja
Belevich, Ilya
Ramo, Olli
Nevzorov, Ilya
Vihinen, Helena
Puhka, Maija
Witkos, Tomasz M.
Lowe, Martin
Vartiainen, Maria K.
Jokitalo, Eija
Title ER sheet persistence is coupled to myosin 1c-regulated dynamic actin filament arrays
Journal name Molecular Biology of the Cell   Check publisher's open access policy
ISSN 1939-4586
1059-1524
Publication date 2014-04-01
Sub-type Article (original research)
DOI 10.1091/mbc.E13-12-0712
Open Access Status File (Publisher version)
Volume 25
Issue 7
Start page 1111
End page 1126
Total pages 16
Place of publication Bethesda, MD United States
Publisher American Society for Cell Biology
Language eng
Abstract The endoplasmic reticulum (ER) comprises a dynamic three-dimensional (3D) network with diverse structural and functional domains. Proper ER operation requires an intricate balance within and between dynamics, morphology, and functions, but how these processes are coupled in cells has been unclear. Using live-cell imaging and 3D electron microscopy, we identify a specific subset of actin filaments localizing to polygons defined by ER sheets and tubules and describe a role for these actin arrays in ER sheet persistence and, thereby, in maintenance of the characteristic network architecture by showing that actin depolymerization leads to increased sheet fluctuation and transformations and results in small and less abundant sheet remnants and a defective ER network distribution. Furthermore, we identify myosin 1c localizing to the ER-associated actin filament arrays and reveal a novel role for myosin 1c in regulating these actin structures, as myosin 1c manipulations lead to loss of the actin filaments and to similar ER phenotype as observed after actin depolymerization. We propose that ER-associated actin filaments have a role in ER sheet persistence regulation and thus support the maintenance of sheets as a stationary subdomain of the dynamic ER network.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Queensland Brain Institute Publications
 
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