Memory T-cell responses to Vibrio cholerae O1 infection

Weil, Ana A., Arifuzzaman, Mohammad, Bhuiyan, Taufiqur R., LaRocque, Regina C., Harris, Aaron M., Kendall, Emily A., Hossain, Azim, Tarique, Abdullah A., Sheikh, Alaullah, Chowdhury, Fahima, Khan, Ashraful I., Murshed, Farhan, Parker, Kenneth C., Banerjee, Kalyan K., Ryan, Edward T., Harris, Jason B., Qadri, Firdausi and Calderwood, Stephen B. (2009) Memory T-cell responses to Vibrio cholerae O1 infection. Infection and Immunity, 77 11: 5090-5096. doi:10.1128/IAI.00793-09

Author Weil, Ana A.
Arifuzzaman, Mohammad
Bhuiyan, Taufiqur R.
LaRocque, Regina C.
Harris, Aaron M.
Kendall, Emily A.
Hossain, Azim
Tarique, Abdullah A.
Sheikh, Alaullah
Chowdhury, Fahima
Khan, Ashraful I.
Murshed, Farhan
Parker, Kenneth C.
Banerjee, Kalyan K.
Ryan, Edward T.
Harris, Jason B.
Qadri, Firdausi
Calderwood, Stephen B.
Title Memory T-cell responses to Vibrio cholerae O1 infection
Journal name Infection and Immunity   Check publisher's open access policy
ISSN 00199567 10985522
Publication date 2009-11-01
Year available 2009
Sub-type Article (original research)
DOI 10.1128/IAI.00793-09
Open Access Status Not yet assessed
Volume 77
Issue 11
Start page 5090
End page 5096
Total pages 7
Language eng
Subject 2405 Parasitology
2404 Microbiology
2403 Immunology
2725 Infectious Diseases
Abstract Vibrio cholerae O1 can cause diarrheal disease that may be life-threatening without treatment. Natural infection results in long-lasting protective immunity, but the role of T cells in this immune response has not been well characterized. In contrast, robust B-cell responses to V. cholerae infection have been observed. In particular, memory B-cell responses to T-cell-dependent antigens persist for at least 1 year, whereas responses to lipopolysaccharide, a T-cell-independent antigen, wane more rapidly after infection. We hypothesize that protective immunity is mediated by anamnestic responses of memory B cells in the gut-associated lymphoid tissue, and T-cell responses may be required to generate and maintain durable memory B-cell responses. In this study, we examined B- and T-cell responses in patients with severe V. cholerae infection. Using the flow cytometric assay of the specific cell-mediated immune response in activated whole blood, we measured antigen-specific T-cell responses using V. cholerae antigens, including the toxin-coregulated pilus (TcpA), a V. cholerae membrane preparation, and the V. cholerae cytolysin/hemolysin (VCC) protein. Our results show that memory T-cell responses develop by day 7 after infection, a time prior to and concurrent with the development of B-cell responses. This suggests that T-cell responses to V. cholerae antigens may be important for the generation and stability of memory B-cell responses. The T-cell proliferative response to VCC was of a higher magnitude than responses observed to other V. cholerae antigens. Copyright
Keyword Immunology
Infectious Diseases
Infectious Diseases
Q-Index Code C1
Q-Index Status Provisional Code
Grant ID U01 AI058935
KO1 TW07144
D43 TW05572
D43 TW005572
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
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Citation counts: TR Web of Science Citation Count  Cited 25 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 27 times in Scopus Article | Citations
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