Rapid phenotyping adult plant resistance to stem rust in wheat grown under controlled conditions

Riaz, Adnan and Hickey, Lee T. (2017). Rapid phenotyping adult plant resistance to stem rust in wheat grown under controlled conditions. In Sambasivam Periyannan (Ed.), Wheat rust diseases: methods and protocols (pp. 183-196) New York, NY, United States: Humana Press. doi:10.1007/978-1-4939-7249-4_16

Author Riaz, Adnan
Hickey, Lee T.
Title of chapter Rapid phenotyping adult plant resistance to stem rust in wheat grown under controlled conditions
Title of book Wheat rust diseases: methods and protocols
Place of Publication New York, NY, United States
Publisher Humana Press
Publication Year 2017
Sub-type Research book chapter (original research)
DOI 10.1007/978-1-4939-7249-4_16
Open Access Status Not yet assessed
Series Methods in Molecular Biology
ISBN 9781493972487
ISSN 1064-3745
Editor Sambasivam Periyannan
Volume number 1659
Chapter number 16
Start page 183
End page 196
Total pages 14
Total chapters 24
Language eng
Subjects 1312 Molecular Biology
1311 Genetics
Abstract/Summary Stem rust (SR) or black rust caused by Puccinia graminis f. sp. tritici is one of the most common diseases of wheat (Triticum aestivum L.) crops globally. Among the various control measures, the most efficient and sustainable approach is the deployment of genetically resistant cultivars. Traditionally, wheat breeding programs deployed genetic resistance in cultivars, but unknowingly this is often underpinned by a single seedling resistance gene, which is readily overcome by the pathogen. Nowadays, adult plant resistance (APR) is a widely adopted form of rust resistance because more durable mechanisms often underpin it. However, only a handful of SR APR genes are available, so breeders currently strive to combine seedling and APR genes. Phenotyping adult wheat plants for resistance to SR typically involves evaluation in the field. But establishing a rust nursery can be challenging, and screening is limited to once a year. This slows down research efforts to isolate new APR genes and breeding of genetically resistant cultivars. In this study, we report a protocol for rapid evaluation of adult wheat plants for resistance to stem rust. We demonstrate the technique by evaluating a panel of 16 wheat genotypes consisting of near isogenic lines (NILs) for known Sr genes (i.e., Sr2, Sr33, Sr45, Sr50, Sr55, Sr57, and Sr58) and three landraces carrying uncharacterized APR from the N. I. Vavilov Institute of Plant Genetic Resources (VIR). The method can be completed in just 10 weeks and involves two inoculations: first conducted at seedling stage and a second at the adult stage (using the same plants). The technique can detect APR, such as that conferred by APR gene Sr2, along with pseudo-black chaff (the morphological marker). Phenotyping can be conducted throughout the year, and is fast and resource efficient. Further, the phenotyping method can be applied to screen breeding populations or germplasm accessions using local or exotic races of SR.
Keyword Adult plant resistance
Controlled environment
Seedling resistance
Speed breeding
Stem rust
Q-Index Code B1
Q-Index Status Provisional Code
Institutional Status UQ

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