Recovery of nearly 8,000 metagenome-assembled genomes substantially expands the tree of life

Parks D.H., Rinke C., Chuvochina M., Chaumeil P.-A., Woodcroft B.J., Evans P.N., Hugenholtz P. and Tyson G.W. (2017) Recovery of nearly 8,000 metagenome-assembled genomes substantially expands the tree of life. Nature Microbiology, 2 11: 1-10. doi:10.1038/s41564-017-0012-7


Author Parks D.H.
Rinke C.
Chuvochina M.
Chaumeil P.-A.
Woodcroft B.J.
Evans P.N.
Hugenholtz P.
Tyson G.W.
Title Recovery of nearly 8,000 metagenome-assembled genomes substantially expands the tree of life
Journal name Nature Microbiology   Check publisher's open access policy
ISSN 2058-5276
Publication date 2017-09-01
Sub-type Article (original research)
DOI 10.1038/s41564-017-0012-7
Open Access Status Not yet assessed
Volume 2
Issue 11
Start page 1
End page 10
Total pages 10
Publisher Nature Publishing Group
Language eng
Subject 2404 Microbiology
2403 Immunology
2402 Applied Microbiology and Biotechnology
1311 Genetics
2726 Microbiology (medical)
1307 Cell Biology
Abstract Challenges in cultivating microorganisms have limited the phylogenetic diversity of currently available microbial genomes. This is being addressed by advances in sequencing throughput and computational techniques that allow for the cultivation-independent recovery of genomes from metagenomes. Here, we report the reconstruction of 7,903 bacterial and archaeal genomes from >1,500 public metagenomes. All genomes are estimated to be ≥50% complete and nearly half are ≥90% complete with ≤5% contamination. These genomes increase the phylogenetic diversity of bacterial and archaeal genome trees by >30% and provide the first representatives of 17 bacterial and three archaeal candidate phyla. We also recovered 245 genomes from the Patescibacteria superphylum (also known as the Candidate Phyla Radiation) and find that the relative diversity of this group varies substantially with different protein marker sets. The scale and quality of this data set demonstrate that recovering genomes from metagenomes provides an expedient path forward to exploring microbial dark matter.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
School of Chemistry and Molecular Biosciences
 
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Created: Tue, 26 Sep 2017, 00:15:25 EST by Web Cron