Isolation and characterization of a Cotesia rubecula bracovirus gene expressed in the lepidopteran Pieris rapae

Glatz, R., Schmidt, O. and Asgari, S. (2004) Isolation and characterization of a Cotesia rubecula bracovirus gene expressed in the lepidopteran Pieris rapae. Journal of General Virology, 85 10: 2873-2882. doi:10.1099/vir.0.80307-0


Author Glatz, R.
Schmidt, O.
Asgari, S.
Title Isolation and characterization of a Cotesia rubecula bracovirus gene expressed in the lepidopteran Pieris rapae
Journal name Journal of General Virology   Check publisher's open access policy
ISSN 0022-1317
1465-2099
Publication date 2004-01-01
Sub-type Article (original research)
DOI 10.1099/vir.0.80307-0
Open Access Status Not Open Access
Volume 85
Issue 10
Start page 2873
End page 2882
Total pages 10
Editor G.L. Smith
Place of publication Reading
Publisher Society of General Microbiology
Language eng
Subject C1
270303 Virology
780105 Biological sciences
0605 Microbiology
Abstract Polydnaviruses are endogenous particles that are crucial for the survival of endoparasitoid wasps, providing active suppression of the immune function of the lepidopteran host in which wasp larvae develop. The Cotesia rubecula bracovirus (CrBV) is unique in that only four gene products are detected in larval host (Pieris rapae) tissues and expression of CrBV genes is transient, occurring between 4 and 12 h post-parasitization. Two of the four genes, CrV1 and CrV3, have been characterized. CrV1 is a secreted glycoprotein that has been implicated in depolymerization of the actin cytoskeleton of host haemocytes, leading to haemocyte inactivation; CrV3 is a multimeric C-type lectin that shares homology with insect immune lectins. Here, a third CrBV-specific gene is described, CrV2, which is expressed in larval P. rapae tissues. CrV2, which is transcribed in haemocytes and fat body cells, has an ORF of 963 bp that produces a glycoprotein of approximately 40 kDa. CrV2 is secreted into haemolymph and appears to be internalized by host haemocytes. CrV2 has a coiled-coil region predicted at its C-terminus, which may be involved in the formation of putative CrV2 trimers that are detected in haemolymph of parasitized host larvae.
Keyword Biotechnology & Applied Microbiology
Virology
Microplitis-demolitor Polydnavirus
C-type Lectin
Manduca-sexta
Pseudoplusia-includens
Tobacco Hornworm
Congregata Polydnavirus
Campoletis-sonorensis
Cdna Cloning
Host Larvae
Protein
Q-Index Code C1

 
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Created: Wed, 15 Aug 2007, 13:07:22 EST