Genomic and in situ investigations of the novel uncultured Chloroflexi associated with 0092 morphotype filamentous bulking in activated sludge

McIlroy, Simon Jon, Karst, Soren Michael, Nierychlo, Marta, Dueholm, Morten Simonsen, Albertsen, Mads, Kirkegaard, Rasmus Hansen, Seviour, Robert James and Nielsen, Per Halkjaer (2016) Genomic and in situ investigations of the novel uncultured Chloroflexi associated with 0092 morphotype filamentous bulking in activated sludge. ISME Journal, 10 9: 2223-2234. doi:10.1038/ismej.2016.14


Author McIlroy, Simon Jon
Karst, Soren Michael
Nierychlo, Marta
Dueholm, Morten Simonsen
Albertsen, Mads
Kirkegaard, Rasmus Hansen
Seviour, Robert James
Nielsen, Per Halkjaer
Title Genomic and in situ investigations of the novel uncultured Chloroflexi associated with 0092 morphotype filamentous bulking in activated sludge
Formatted title
Genomic and in situ investigations of the novel uncultured Chloroflexi associated with 0092 morphotype filamentous bulking in activated sludge
Journal name ISME Journal   Check publisher's open access policy
ISSN 1751-7362
1751-7370
Publication date 2016-09-01
Sub-type Article (original research)
DOI 10.1038/ismej.2016.14
Open Access Status Not yet assessed
Volume 10
Issue 9
Start page 2223
End page 2234
Total pages 12
Place of publication London, United Kingdom
Publisher Nature Publishing Group
Language eng
Abstract Overgrowth of filamentous bacteria in activated sludge wastewater treatment plants (WWTPs) leads to impaired sludge settleability, a condition known as bulking, which is a common operational problem worldwide. Filaments with the Eikelboom 0092 morphotype are commonly associated with such bulking episodes. Members of the uncultured B45 phylotype, which is embraced within the phylum Chloroflexi, were recently shown to exhibit this morphology. Although these organisms are among the most abundant populations recorded in activated sludge processes, nothing is known about their metabolic characteristics. In this study, a genome sequence, representing the B45 phylotype, was retrieved from a metagenome generated from an activated sludge WWTP. The genome consisted of two chromosomes and one plasmid, which were 4.0, 1.0 and 0.04 Mbps in size, respectively. A metabolic model was constructed for this organism, based on annotation of its genome, showing its ability to generate energy by respiration, utilizing oxygen, nitrite or nitrous oxide as electron acceptors, or by fermentation of sugars. The ability of B45 members to ferment sugars under anaerobic conditions was validated in situ with microautoradiography—fluorescence in situ hybridization. The provisional name of ‘Candidatus Promineofilum breve’ is proposed for this species. This study represents the first detailed information on an uncultured genus of filamentous organisms from activated sludge.
Keyword Targeted oligonucleotide probes
Biological phosphorus removal
Water treatment plants
C nitrite reductase
Waste water
Phylum chloroflexi
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
School of Chemistry and Molecular Biosciences
 
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