Genomic insights into members of the candidate phylum Hyd24-12 common in mesophilic anaerobic digesters

Kirkegaard, Rasmus Hansen, Dueholm, Morten Simonsen, McIlroy, Simon Jon, Nierychlo, Marta, Karst, Soren Michael, Albertsen, Mads and Nielsen, Per Halkjaer (2016) Genomic insights into members of the candidate phylum Hyd24-12 common in mesophilic anaerobic digesters. ISME Journal, 10 10: 2352-2364. doi:10.1038/ismej.2016.43


Author Kirkegaard, Rasmus Hansen
Dueholm, Morten Simonsen
McIlroy, Simon Jon
Nierychlo, Marta
Karst, Soren Michael
Albertsen, Mads
Nielsen, Per Halkjaer
Title Genomic insights into members of the candidate phylum Hyd24-12 common in mesophilic anaerobic digesters
Journal name ISME Journal   Check publisher's open access policy
ISSN 1751-7362
1751-7370
Publication date 2016-10-01
Sub-type Article (original research)
DOI 10.1038/ismej.2016.43
Open Access Status Not yet assessed
Volume 10
Issue 10
Start page 2352
End page 2364
Total pages 13
Place of publication London, United Kingdom
Publisher Nature Publishing Group
Language eng
Subject 2404 Microbiology
1105 Ecology, Evolution, Behavior and Systematics
Abstract Members of the candidate phylum Hyd24-12 are globally distributed, but no genomic information or knowledge about their morphology, physiology or ecology is available. In this study, members of the Hyd24-12 lineage were shown to be present and abundant in full-scale mesophilic anaerobic digesters at Danish wastewater treatment facilities. In some samples, a member of the Hyd24-12 lineage was one of the most abundant genus-level bacterial taxa, accounting for up to 8% of the bacterial biomass. Three closely related and near-complete genomes were retrieved using metagenome sequencing of full-scale anaerobic digesters. Genome annotation and metabolic reconstruction showed that they are Gram-negative bacteria likely involved in acidogenesis, producing acetate and hydrogen from fermentation of sugars, and may play a role in the cycling of sulphur in the digesters. Fluorescence in situ hybridization revealed single rod-shaped cells dispersed within the flocs. The genomic information forms a foundation for a more detailed understanding of their role in anaerobic digestion and provides the first insight into a hitherto undescribed branch in the tree of life.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
School of Chemistry and Molecular Biosciences
 
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