Caspofungin on ARGET-ATRP grafted PHEMA polymers: enhancement and selectivity of prevention of attachment of Candida albicans

Michl, Thomas D., Giles, Carla, Mocny, Piotr, Futrega, Kathryn, Doran, Michael R., Klok, Harm-Anton, Griesser, Hans J. and Coad, Bryan R. (2017) Caspofungin on ARGET-ATRP grafted PHEMA polymers: enhancement and selectivity of prevention of attachment of Candida albicans. Biointerphases, 12 5: . doi:10.1116/1.4986054


Author Michl, Thomas D.
Giles, Carla
Mocny, Piotr
Futrega, Kathryn
Doran, Michael R.
Klok, Harm-Anton
Griesser, Hans J.
Coad, Bryan R.
Title Caspofungin on ARGET-ATRP grafted PHEMA polymers: enhancement and selectivity of prevention of attachment of Candida albicans
Formatted title
Caspofungin on ARGET-ATRP grafted PHEMA polymers: enhancement and selectivity of prevention of attachment of Candida albicans
Journal name Biointerphases   Check publisher's open access policy
ISSN 1559-4106
1934-8630
Publication date 2017-12-01
Year available 2017
Sub-type Article (original research)
DOI 10.1116/1.4986054
Open Access Status Not yet assessed
Volume 12
Issue 5
Total pages 11
Place of publication Melville, NY, United States
Publisher A I P Publishing LLC
Language eng
Abstract There is a need for coatings for biomedical devices and implants that can prevent the attachment of fungal pathogens while allowing human cells and tissue to appose without cytotoxicity. Here, the authors study whether a poly(2-hydroxyethylmethacrylate) (PHEMA) coating can suppress attachment and biofilm formation by Candida albicans and whether caspofungin terminally attached to surface-tethered polymeric linkers can provide additional benefits. The multistep coating scheme first involved the plasma polymerization of ethanol, followed by the attachment of a-bromoisobutyryl bromide (BiBB) onto surface hydroxyl groups of the plasma polymer layer. Polymer chains were grafted using surface initiated activators regenerated by electron transfer atom transfer radical polymerization with 2-hydroxyethylmethacrylate, yielding PHEMA layers with a dry thickness of up to 89 nm in 2 h. Hydroxyl groups of PHEMA were oxidized to aldehydes using the Albright-Goldman reaction, and caspofungin was covalently immobilized onto them using reductive amination. While the PHEMA layer by itself reduced the growth of C. albicans biofilms by log 1.4, the addition of caspofungin resulted in a marked further reduction by>4 log units to below the threshold of the test. The authors have confirmed that the predominant mechanism of action is caused by antifungal drug molecules that are covalently attached to the surface, rather than out-diffusing from the coating. The authors confirm the selectivity of surface-attached caspofungin in eliminating fungal, not mammalian cells by showing no measurable toxicity toward the myeloid leukaemia suspension cell line KG-1a. VC (C) 2017 American Vacuum Society.
Keyword General biochemistry, genetics and molecular biology
General physics and astronomy
General materials science
General chemistry
Q-Index Code C1
Q-Index Status Provisional Code
Grant ID DP150101674
APP1066647
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Mater Research Institute-UQ (MRI-UQ)
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