The Epstein-Barr virus nuclear antigen-6 protein co-localizes with EBNA-3 and survival of motor neurons protein

Krauer, Kenia G., Buck, Marion, Belzer, Deanna K., Flanagan, James, Chojnowski, Grace M. and Sculley, Tom B. (2004) The Epstein-Barr virus nuclear antigen-6 protein co-localizes with EBNA-3 and survival of motor neurons protein. Virology, 318 1: 280-294. doi:10.1016/j.virol.2003.09.032


Author Krauer, Kenia G.
Buck, Marion
Belzer, Deanna K.
Flanagan, James
Chojnowski, Grace M.
Sculley, Tom B.
Title The Epstein-Barr virus nuclear antigen-6 protein co-localizes with EBNA-3 and survival of motor neurons protein
Journal name Virology   Check publisher's open access policy
ISSN 0042-6822
Publication date 2004-01-01
Sub-type Article (original research)
DOI 10.1016/j.virol.2003.09.032
Open Access Status Not Open Access
Volume 318
Issue 1
Start page 280
End page 294
Total pages 15
Editor R. Lamb
Place of publication San Diego, U.S.A.
Publisher Academic Press
Language eng
Subject C1
320305 Medical Biochemistry - Proteins and Peptides
730108 Cancer and related disorders
Abstract The Epstein-Barr virus nuclear antigen (EBNA)-6 protein is essential for Epstein-Barr virus (EBV)-induced immortalization of primary human B-lymphocytes in vitro. In this study, fusion proteins of EBNA-6 with green fluorescent protein (GFP) have been used to characterize its nuclear localization and organization within the nucleus. EBNA-6 associates with nuclear structures and in immunofluorescence demonstrate a punctate staining pattern. Herein, we show that the association of EBNA-6 with these nuclear structures was maintained throughout the cell cycle and with the use of GFP-E6 deletion mutants, that the region amino acids 733-808 of EBNA-6 contains a domain that can influence the association of EBNA-6 with these nuclear structures. Co-immunofluorescence and confocal analyses demonstrated that EBNA-6 and EBNA-3 co-localize in the nucleus of cells. Expression of EBNA-6, but not EBNA-3, caused a redistribution of nuclear survival of motor neurons protein (SMN) to the EBNA-6 containing nuclear structures resulting in co-localization of SMN with EBNA-6. (C) 2003 Elsevier Inc. All rights reserved.
Keyword Virology
Ebv
Ebna-6
Ebna-3
Nuclear Localization
Nuclear Structures
Smn
Gfp
Gems
Dead Box Protein
Prothymosin-alpha
Binding Protein
Coiled Bodies
Gene-product
J-kappa
3c
Transcription
Localization
Cells
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2005 Higher Education Research Data Collection
School of Medicine Publications
 
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