Molecular regulation of muscle mass in developing Blonde d’Aquitaine foetuses compared to Charolais

Cassar-Malek, Isabelle , Boby, Céline , Picard, Brigitte, Reverter, Antonio and Hudson, Nicholas J. (2017) Molecular regulation of muscle mass in developing Blonde d’Aquitaine foetuses compared to Charolais. Biology Open, 6 10: 1483-1492. doi:10.1242/bio.024950


Author Cassar-Malek, Isabelle
Boby, Céline
Picard, Brigitte
Reverter, Antonio
Hudson, Nicholas J.
Title Molecular regulation of muscle mass in developing Blonde d’Aquitaine foetuses compared to Charolais
Journal name Biology Open   Check publisher's open access policy
ISSN 2046-6390
Publication date 2017-08-24
Year available 2017
Sub-type Article (original research)
DOI 10.1242/bio.024950
Open Access Status DOI
Volume 6
Issue 10
Start page 1483
End page 1492
Total pages 34
Place of publication Cambridge, United Kingdom
Publisher The Company of Biologists
Language eng
Subject 1300 Biochemistry, Genetics and Molecular Biology
1100 Agricultural and Biological Sciences
Abstract The Blonde d’Aquitaine (BA) is a French cattle breed with enhanced muscularity, partly attributable to a MSTN mutation. The BA m. Semitendinosus has a faster muscle fibre isoform phenotype comprising a higher proportion of fast type IIX fibres compared to age-matched Charolais (CH). To better understand the molecular network of modifications in BA compared to CH muscle, we assayed the transcriptomes of the m. Semitendinosus at 110, 180, 210 and 260 days postconception (dpc). We used a combination of differential expression (DE) and regulatory impact factors (RIF) to compare and contrast muscle gene expression between the breeds. Prominently developmentally regulated genes in both breeds reflected the replacement of embryonic myosin isoforms (MYL4, MYH3) with adult isoforms (MYH1) and the upregulation of mitochondrial metabolism (CKMT2, AGXT2L1) in preparation for birth. However, the transition to a fast, glycolytic muscle phenotype in the MSTN mutant BA is detectable through downregulation of various slow twitch subunits (TNNC1, MYH7, TPM3, CSRP3) beyond 210 dpc, and a small but consistent genome-wide reduction in mRNA encoding the mitoproteome. Across the breeds, NRIP2 is the regulatory gene possessing a network change most similar to that of MSTN.
Keyword Genomics
Mitochondria
Myogenesis
Myostatin
Skeletal muscle
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
School of Agriculture and Food Sciences
 
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Created: Wed, 13 Sep 2017, 10:20:50 EST by Nick Hudson on behalf of School of Agriculture and Food Sciences