Metabolomics and genomics combine to unravel the pathway for the presence of fragrance in rice

Daygon, Venea Dara, Calingacion, Mariafe, Forster, Louise C., De Voss, James J., Schwartz, Brett D., Ovenden, Ben, Alonso, David E., McCouch, Susan R., Garson, Mary J. and Fitzgerald, Melissa A. (2017) Metabolomics and genomics combine to unravel the pathway for the presence of fragrance in rice. Scientific Reports, 7 1: 8767-8767. doi:10.1038/s41598-017-07693-9


Author Daygon, Venea Dara
Calingacion, Mariafe
Forster, Louise C.
De Voss, James J.
Schwartz, Brett D.
Ovenden, Ben
Alonso, David E.
McCouch, Susan R.
Garson, Mary J.
Fitzgerald, Melissa A.
Title Metabolomics and genomics combine to unravel the pathway for the presence of fragrance in rice
Journal name Scientific Reports   Check publisher's open access policy
ISSN 2045-2322
Publication date 2017-08-18
Sub-type Article (original research)
DOI 10.1038/s41598-017-07693-9
Open Access Status DOI
Volume 7
Issue 1
Start page 8767
End page 8767
Total pages 12
Place of publication London, United Kingdom
Publisher Nature Publishing Group
Language eng
Subject 1000 General
Abstract Since it was first characterised in 1983, 2-acetyl-1-pyrroline (2AP) has been considered to be the most important aroma compound in rice. In this study, we show four other amine heterocycles: 6-methyl, 5-oxo-2,3,4,5-tetrahydropyridine (6M5OTP), 2-acetylpyrrole, pyrrole and 1-pyrroline, that correlate strongly with the production of 2AP, and are present in consistent proportions in a set of elite aromatic rice varieties from South East Asia and Australia as well as in a collection of recombinant inbred lines (RILs) derived from indica Jasmine-type varieties, Australian long grain varieties (temperate japonica) and Basmati-type rice (Grp V). These compounds were detected through untargeted metabolite profiling by two-dimensional gas chromatography-time-of-flight mass spectrometry (GC × GC-TOF-MS), and their identity were confirmed by comparison with authentic standards analysed using gas chromatography mass spectrometry (GC-MS) and High Resolution GC × GC-TOF-MS (GC × GC HRT-4D). Genome-wide association analysis indicates that all compounds co-localised with a single quantitative trait locus (QTL) that harbours the FGR gene responsible for the production of GABA. Together, these data provide new insights into the production of 2AP, and evidence for understanding the pathway leading to the accumulation of aroma in fragrant rice.
Keyword Multidisciplinary
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
School of Agriculture and Food Sciences
School of Chemistry and Molecular Biosciences
 
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