Transforming growth factor-β1 signaling promotes epithelial-mesenchymal transition-like phenomena, cell motility, and cell invasion in synovial sarcoma cells

Qi, Yan, Wang, Ning, He, Yonglai, Zhang, Jun, Zou, Hong, Zhang, Wenjie, Gu, Wenyi, Huang, Yalan, Lian, Xiaojuan, Hu, Jianming, Zhao, Jin, Cui, Xiaobin, Pang, Lijuan and Li, Feng (2017) Transforming growth factor-β1 signaling promotes epithelial-mesenchymal transition-like phenomena, cell motility, and cell invasion in synovial sarcoma cells. PLoS One, 12 8: e0182680-e0182680. doi:10.1371/journal.pone.0182680


Author Qi, Yan
Wang, Ning
He, Yonglai
Zhang, Jun
Zou, Hong
Zhang, Wenjie
Gu, Wenyi
Huang, Yalan
Lian, Xiaojuan
Hu, Jianming
Zhao, Jin
Cui, Xiaobin
Pang, Lijuan
Li, Feng
Title Transforming growth factor-β1 signaling promotes epithelial-mesenchymal transition-like phenomena, cell motility, and cell invasion in synovial sarcoma cells
Journal name PLoS One   Check publisher's open access policy
ISSN 1932-6203
Publication date 2017-08-22
Year available 2017
Sub-type Article (original research)
DOI 10.1371/journal.pone.0182680
Open Access Status DOI
Volume 12
Issue 8
Start page e0182680
End page e0182680
Total pages 12
Place of publication San Francisco, CA, United States
Publisher Public Library of Science
Language eng
Abstract The epithelial-to-mesenchymal transition (EMT) and the reverse process (the mesenchymal-to-epithelial transition [MET]) have been shown to be associated with tumor cell invasion and metastasis in different carcinomas. The EMT and MET have recently been shown to play a key role in the pathogenic processes of sarcomas, which are completely different from those of carcinomas. However, the definitive roles of the EMT in the tumorigenesis of synovial sarcomas remain unknown. Here, we explored whether transforming growth factor (TGF)-β signaling, an important oncogenic event in synovial sarcoma, modulates tumor cell characteristics related to the EMT, such as cell adhesion, migration, invasion, and proliferation. Interestingly, we found that TGF-β1 induced tumor cell activation, resulting in a tendency to aggregate and biphasic-like features. TGF-β1 also caused downregulation of E-cadherin and subsequent upregulation of N-cadherin, Snail, and Slug, which are responsible for EMT-like phenomena and increased cell motility and invasion. To further investigate the roles of TGF-β1 in the EMT, we established a SW982 cell line with stable TGF-β1 inhibition viaSB431542.These cells exhibited significantly decreased motility, migration, and proliferation (P = 0.001). Taken together, our data demonstrated that alterations in the TGF-β1/Smad signaling pathway could regulate the expression of EMT-related factors and the EMT process, resulting in changes in tumor cell invasion, migration, and proliferation in synovial sarcoma cells. These results may provide a important insights into therapeutic interventions and contribute to the present understanding of tumor progression in patients.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
Australian Institute for Bioengineering and Nanotechnology Publications
 
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