Evidence for newly generated interneurons in the basolateral amygdala of adult mice

Jhaveri, D. J., Tedoldi, A., Hunt, S., Sullivan, R., Watts, N. R., Power, J. M., Bartlett, P. F. and Sah, P. (2017) Evidence for newly generated interneurons in the basolateral amygdala of adult mice. Molecular Psychiatry, 23 3: 521-532. doi:10.1038/mp.2017.134

Author Jhaveri, D. J.
Tedoldi, A.
Hunt, S.
Sullivan, R.
Watts, N. R.
Power, J. M.
Bartlett, P. F.
Sah, P.
Title Evidence for newly generated interneurons in the basolateral amygdala of adult mice
Journal name Molecular Psychiatry   Check publisher's open access policy
ISSN 1359-4184
Publication date 2017-08-15
Year available 2018
Sub-type Article (original research)
DOI 10.1038/mp.2017.134
Open Access Status Not yet assessed
Volume 23
Issue 3
Start page 521
End page 532
Total pages 12
Place of publication London, United Kingdom
Publisher Nature Publishing Group
Language eng
Abstract New neurons are continually generated from the resident populations of precursor cells in selective niches of the adult mammalian brain such as the hippocampal dentate gyrus and the olfactory bulb. However, whether such cells are present in the adult amygdala, and their neurogenic capacity, is not known. Using the neurosphere assay, we demonstrate that a small number of precursor cells, the majority of which express Achaete-scute complex homolog 1 (Ascl1), are present in the basolateral amygdala (BLA) of the adult mouse. Using neuron-specific Thy1-YFP transgenic mice, we show that YFP+ cells in BLA-derived neurospheres have a neuronal morphology, co-express the neuronal marker βIII-tubulin, and generate action potentials, confirming their neuronal phenotype. In vivo, we demonstrate the presence of newly generated BrdU-labeled cells in the adult BLA, and show that a proportion of these cells co-express the immature neuronal marker doublecortin (DCX). Furthermore, we reveal that a significant proportion of GFP+ neurons (~23%) in the BLA are newly generated (BrdU+) in DCX-GFP mice, and using whole-cell recordings in acute slices we demonstrate that the GFP+ cells display electrophysiological properties that are characteristic of interneurons. Using retrovirus-GFP labeling as well as the Ascl1CreERT2 mouse line, we further confirm that the precursor cells within the BLA give rise to mature and functional interneurons that persist in the BLA for at least 8 weeks after their birth. Contextual fear conditioning has no effect on the number of neurospheres or BrdU-labeled cells in the BLA, but produces an increase in hippocampal cell proliferation. These results demonstrate that neurogenic precursor cells are present in the adult BLA, and generate functional interneurons, but also show that their activity is not regulated by an amygdala-dependent learning paradigm.Molecular Psychiatry advance online publication, 15 August 2017; doi:10.1038/mp.2017.134.
Keyword Long-Term Potentiation
Dentate Granule Cells
Neural Stem-Cell
Hippocampal Neurogenesis
Progenitor Cells
Rat Amygdala
Q-Index Code C1
Q-Index Status Provisional Code
Grant ID 301204
Institutional Status UQ
Additional Notes Advance online publication 15 August 2017

Document type: Journal Article
Sub-type: Article (original research)
Collections: Mater Research Institute-UQ (MRI-UQ)
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Queensland Brain Institute Publications
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Created: Mon, 04 Sep 2017, 15:12:33 EST by Kirstie Asmussen on behalf of Queensland Brain Institute