In vitro comparison of the dermal penetration of three different topical formulations containing lasalocid

Knight, Evie C., Trott, Darren J., Page, Stephen W., Garg, Sanjay, Zhang, Qian, Song, Yunmei, Ebrahimie, Esmaeil, Mills, Paul C. and Shipstone, Michael A. (2017) In vitro comparison of the dermal penetration of three different topical formulations containing lasalocid. Veterinary Dermatology, 28 4: 342-e74. doi:10.1111/vde.12379


Author Knight, Evie C.
Trott, Darren J.
Page, Stephen W.
Garg, Sanjay
Zhang, Qian
Song, Yunmei
Ebrahimie, Esmaeil
Mills, Paul C.
Shipstone, Michael A.
Title In vitro comparison of the dermal penetration of three different topical formulations containing lasalocid
Formatted title
In vitro comparison of the dermal penetration of three different topical formulations containing lasalocid
Journal name Veterinary Dermatology   Check publisher's open access policy
ISSN 0959-4493
1365-3164
Publication date 2017-08-01
Year available 2017
Sub-type Article (original research)
DOI 10.1111/vde.12379
Open Access Status Not yet assessed
Volume 28
Issue 4
Start page 342
End page e74
Total pages 7
Place of publication Chichester, West Sussex United Kingdom
Publisher Wiley-Blackwell Publishing
Language eng
Subject 3400 Veterinary
Abstract Background: Topical antimicrobial preparations are of utmost importance in treating suspected and confirmed meticillin-resistant Staphylococcus pseudintermedius (MRSP) infections due to the increasing incidence of widespread resistance to systemic antimicrobials. Lasalocid is active against MRSP in vitro and this may become an important topical antimicrobial for the treatment of canine pyoderma. Hypothesis/Objectives: To determine effects of various formulation types on penetration and retention of lasalocid applied to canine skin in vitro. Animals: Normal canine skin was collected from the thorax of five dogs that had been euthanized on the basis of health and/or intractable behavioural issues. Methods: Solution, lotion and ointment containing 2% lasalocid were applied to ex vivo canine skin. Transdermal penetration was assessed for a 24 h period and retention of lasalocid was assessed at the conclusion of the study. Results: The solution had significantly higher skin retention of lasalocid and proportion of applied dose retained in skin than lotion and ointment (Tukey–Kramer Honest Significant Difference test, P < 0.01). Lasalocid could not be detected in the receptor fluid of any Franz cell at any time point. Conclusions and clinical importance: Lasalocid was not identified in the receptor fluid of any sample, indicating that systemic absorption of the active ingredient in vivo is unlikely. Lasalocid may be useful in the treatment of MRSP infections if in vivo studies support safety and efficacy.
Formatted abstract
Background

Topical antimicrobial preparations are of utmost importance in treating suspected and confirmed meticillin-resistant Staphylococcus pseudintermedius (MRSP) infections due to the increasing incidence of widespread resistance to systemic antimicrobials. Lasalocid is active against MRSP in vitro and this may become an important topical antimicrobial for the treatment of canine pyoderma.

Hypothesis/Objectives

To determine effects of various formulation types on penetration and retention of lasalocid applied to canine skin in vitro.

Animals

Normal canine skin was collected from the thorax of five dogs that had been euthanized on the basis of health and/or intractable behavioural issues.

Methods

Solution, lotion and ointment containing 2% lasalocid were applied to ex vivo canine skin. Transdermal penetration was assessed for a 24 h period and retention of lasalocid was assessed at the conclusion of the study.

Results

The solution had significantly higher skin retention of lasalocid and proportion of applied dose retained in skin than lotion and ointment (Tukey–Kramer Honest Significant Difference test, P < 0.01). Lasalocid could not be detected in the receptor fluid of any Franz cell at any time point.

Conclusions and clinical importance

Lasalocid was not identified in the receptor fluid of any sample, indicating that systemic absorption of the active ingredient in vivo is unlikely. Lasalocid may be useful in the treatment of MRSP infections if in vivo studies support safety and efficacy.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
School of Veterinary Science Publications
 
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Created: Mon, 28 Aug 2017, 01:01:01 EST by Web Cron on behalf of School of Veterinary Science